A Meta-Analysis to Determine the State of Biological Control of Aphanomyces Root Rot

Ashebir T Godebo; Naomi Marie J Wee; Christopher K Yost; Fran L Walley; James J Germida

doi:10.3389/fmolb.2021.777042

A Meta-Analysis to Determine the State of Biological Control of Aphanomyces Root Rot

Front Mol Biosci. 2022 Feb 2:8:777042. doi: 10.3389/fmolb.2021.777042. eCollection 2021.

Authors

Ashebir T Godebo¹, Naomi Marie J Wee^{2

3}, Christopher K Yost^{2

3}, Fran L Walley¹, James J Germida¹

Affiliations

¹ Department of Soil Science, University of Saskatchewan, Saskatoon, SK, Canada.
² Department of Biology, University of Regina, Regina, SK, Canada.
³ Institute for Microbial Systems and Society, University of Regina, Regina, SK, Canada.

Abstract

The increasing incidence and prevalence of the pathogen Aphanomyces euteiches in various pulse-growing regions worldwide necessitates the development of effective management strategies, including biological control agents. Numerous labs have undertaken research examining biological control methods to evaluate aphanomyces root rot suppression in multistep processes that include isolation of inhibitory organisms, lab assays, growth chamber assays, and field trials. Given the emergence of various biocontrol agents and the need to mitigate aphanomyces yield losses, we have undertaken a meta-analysis approach to analyze the effectiveness of biocontrol agents in relation to application method, biocontrol agent richness, biocontrol agent type, the type of study, and reporting system-oriented moderator variables. An effect size, calculated as a natural log response ratio, resulted in a summary weighted mean of -0.411, suggesting the overall effectiveness of biocontrol agents (p < .001). Aphanomyces root rot suppression using biological treatments showed significant heterogeneity for all moderator variables, confirming that the studies do not share a common effect size and the use of a random effect model was appropriate. Across studies, meta-analyses revealed that soil amendments, biocontrol agent application as a seed coating and suspension, bacterial and fungal biocontrol agents, mixed applications, growth chamber and field studies, and qualitative and quantitative reporting systems were all associated with significantly positive outcomes for aphanomyces root rot suppression. Our findings suggest that there is potential promise for biological control of aphanomyces root rot, and more field trials need to be conducted to demonstrate the efficacy level observed under growth chamber conditions. Moreover, we identified a lack of detailed understanding of the mechanism(s) of biological control of aphanomyces root rot as a research priority.

Keywords: Aphanomyces euteiches; aphanomyces root rot; biocontrol; meta-analysis; microbial biocontrol agents.

Publication types

Review