Generation and characterization of human-derived iPSC lines from one pair of dizygotic twins discordant for schizophrenia

Peiyan Ni; Huiyao Wang; Jia Cai; Junzhe Ran; Youhui Jiang; Liansheng Zhao; Jinxue Wei; Rongjun Ni; Yingcheng Wang; Xiaohong Ma; Qiang Wang; Wanjun Guo; Tao Li

doi:10.1016/j.scr.2022.102710

Generation and characterization of human-derived iPSC lines from one pair of dizygotic twins discordant for schizophrenia

Stem Cell Res. 2022 Apr:60:102710. doi: 10.1016/j.scr.2022.102710. Epub 2022 Feb 15.

Authors

Peiyan Ni¹, Huiyao Wang¹, Jia Cai¹, Junzhe Ran¹, Youhui Jiang¹, Liansheng Zhao¹, Jinxue Wei¹, Rongjun Ni¹, Yingcheng Wang¹, Xiaohong Ma¹, Qiang Wang¹, Wanjun Guo¹, Tao Li²

Affiliations

¹ The Psychiatric Laboratory and Mental Health Center, West China Hospital, Sichuan University, Chengdu 610041, China.
² Department of Neurobiology, Affiliated Mental Health Center & Hangzhou Seventh People's Hospital, Zhejiang University School of Medicine, Hangzhou 310013, Zhejiang, China; NHC and CAMS Key Laboratory of Medical Neurobiology, MOE Frontier Science Center for Brain Science and Brain-machine Integration, School of Brain Science and Brain Medicine, Zhejiang University, Hangzhou, Zhejiang, China. Electronic address: litaozjusc@zju.edu.cn.

PMID: 35182860
DOI: 10.1016/j.scr.2022.102710

Abstract

Schizophrenia (SCZ) is a debilitating neurodevelopmental disorder with a high heritability. In this study, peripheral blood mononuclear cells (PBMCs) were donated by a pair of dizygotic twins. The female was clinically diagnosed as SCZ by Diagnostic and Statistical Manual of Mental Disorders IV (DSM-IV) criteria, and her unaffected male sibling was healthy control. Induced pluripotent stem cells (iPSCs) were established using Episomal vectors carrying reprograming factors OCT4, SOX2, NANOG, LIN28, c-MYC, KLF4, and SV40LT. These lines with normal karyotype highly expressed pluripotency markers and are capable to differentiate into derivatives of three germ layers. Both lines are negative of mycoplasma.

Keywords: Dizygotic twins; Induced pluripotent stem cells (iPSCs); Schizophrenia (SCZ).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation
Female
Humans
Induced Pluripotent Stem Cells*
Kruppel-Like Factor 4
Leukocytes, Mononuclear
Male
Schizophrenia* / genetics
Twins, Dizygotic

Substances

Kruppel-Like Factor 4