Preparation and use of wireless reprogrammable multilateral optogenetic devices for behavioral neuroscience

Yiyuan Yang; Mingzheng Wu; Amy J Wegener; Abraham Vázquez-Guardado; Andrew I Efimov; Ferrona Lie; Taoyi Wang; Yuhang Ma; Anthony Banks; Zhengwei Li; Zhaoqian Xie; Yonggang Huang; Cameron H Good; Yevgenia Kozorovitskiy; John A Rogers

doi:10.1038/s41596-021-00672-5

Preparation and use of wireless reprogrammable multilateral optogenetic devices for behavioral neuroscience

Nat Protoc. 2022 Apr;17(4):1073-1096. doi: 10.1038/s41596-021-00672-5. Epub 2022 Feb 16.

Authors

Yiyuan Yang^#¹, Mingzheng Wu^#², Amy J Wegener^#^{3

4}, Abraham Vázquez-Guardado^#^{5

6}, Andrew I Efimov⁷, Ferrona Lie⁸, Taoyi Wang⁹, Yuhang Ma¹⁰, Anthony Banks^{5

6

8

11}, Zhengwei Li⁵, Zhaoqian Xie^{12

13

14}, Yonggang Huang^{1

5

15

16}, Cameron H Good^{17

18

19}, Yevgenia Kozorovitskiy^{20

21}, John A Rogers^{22

23

24

25

26

27

28

29

30

31}

Affiliations

¹ Department of Mechanical Engineering, Northwestern University, Evanston, IL, USA.
² Department of Neurobiology, Northwestern University, Evanston, IL, USA.
³ US Army Research Laboratory, Aberdeen Proving Ground, MD, USA.
⁴ US Army Medical Research Institute of Chemical Defense, Aberdeen Proving Ground, MD, USA.
⁵ Center for Bio-Integrated Electronics, Northwestern University, Evanston, IL, USA.
⁶ Querrey Simpson Institute for Bioelectronics, Northwestern University, Evanston, IL, USA.
⁷ Department of Biomedical Engineering, Northwestern University, Evanston, IL, USA.
⁸ Neurolux Inc., Evanston, IL, USA.
⁹ Department of Precision Instrument, Tsinghua University, Beijing, China.
¹⁰ School of Chemical Engineering and Technology, Tianjin University, Tianjin, China.
¹¹ Feinberg School of Medicine, Northwestern University, Evanston, IL, USA.
¹² State Key Laboratory of Structural Analysis for Industrial Equipment, Department of Engineering Mechanics, Dalian University of Technology, Dalian, China.
¹³ Department of Engineering Mechanics, Dalian University of Technology, Dalian, China.
¹⁴ Ningbo Institute of Dalian University of Technology, Ningbo, China.
¹⁵ Department of Civil and Environmental Engineering, Northwestern University, Evanston, IL, USA.
¹⁶ Department of Materials Science and Engineering, Northwestern University, Evanston, IL, USA.
¹⁷ US Army Research Laboratory, Aberdeen Proving Ground, MD, USA. cameron.h.good@gmail.com.
¹⁸ US Army Medical Research Institute of Chemical Defense, Aberdeen Proving Ground, MD, USA. cameron.h.good@gmail.com.
¹⁹ Center for Bio-Integrated Electronics, Northwestern University, Evanston, IL, USA. cameron.h.good@gmail.com.
²⁰ Department of Neurobiology, Northwestern University, Evanston, IL, USA. yevgenia.kozorovitskiy@northwestern.edu.
²¹ Chemistry of Life Processes Institutes, Northwestern University, Evanston, IL, USA. yevgenia.kozorovitskiy@northwestern.edu.
²² Department of Mechanical Engineering, Northwestern University, Evanston, IL, USA. jrogers@northwestern.edu.
²³ Querrey Simpson Institute for Bioelectronics, Northwestern University, Evanston, IL, USA. jrogers@northwestern.edu.
²⁴ Department of Biomedical Engineering, Northwestern University, Evanston, IL, USA. jrogers@northwestern.edu.
²⁵ Neurolux Inc., Evanston, IL, USA. jrogers@northwestern.edu.
²⁶ Feinberg School of Medicine, Northwestern University, Evanston, IL, USA. jrogers@northwestern.edu.
²⁷ Department of Materials Science and Engineering, Northwestern University, Evanston, IL, USA. jrogers@northwestern.edu.
²⁸ Department of Chemistry, Northwestern University, Evanston, IL, USA. jrogers@northwestern.edu.
²⁹ Department of Neurological Surgery, Northwestern University, Evanston, IL, USA. jrogers@northwestern.edu.
³⁰ Department of Electrical and Computer Engineering, Northwestern University, Evanston, IL, USA. jrogers@northwestern.edu.
³¹ Department of Computer Science, Northwestern University, Evanston, IL, USA. jrogers@northwestern.edu.

^# Contributed equally.

Abstract

Wireless battery-free optogenetic devices enable behavioral neuroscience studies in groups of animals with minimal interference to natural behavior. Real-time independent control of optogenetic stimulation through near-field communication dramatically expands the realm of applications of these devices in broad contexts of neuroscience research. Dissemination of these tools with advanced functionalities to the neuroscience community requires protocols for device manufacturing and experimental implementation. This protocol describes detailed procedures for fabrication, encapsulation and implantation of recently developed advanced wireless devices in head- and back-mounted forms. In addition, procedures for standard implementation of experimental systems in mice are provided. This protocol aims to facilitate the application of wireless optogenetic devices in advanced optogenetic experiments involving groups of freely moving rodents and complex environmental designs. The entire protocol lasts ~3-5 weeks.

Publication types

Review
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Mice
Neurosciences*
Optogenetics* / methods
Wireless Technology

Abstract

Publication types

MeSH terms

Grants and funding