Multiplexed quantitative neuropeptidomics via DiLeu isobaric tagging

Methods Enzymol. 2022:663:235-257. doi: 10.1016/bs.mie.2021.10.011. Epub 2021 Dec 7.

Abstract

Neuropeptides are key signaling molecules in many pathways and can serve as potential biomarkers or therapeutics. Mass spectrometry has emerged as a powerful tool for studying neuropeptides with high sensitivity and accuracy. Isobaric tagging can further enhance this method by improving throughput and reducing sampling needs. In this chapter, we discuss the benefits and limitations of using isobaric tags to analyze neuropeptides. Methods for optimizing the data acquisition are also presented to enable a greater number of neuropeptides to be identified and quantified when using isobaric tags, specifically N,N-dimethyl leucine (DiLeu).

Keywords: Data-dependent acquisition; DiLeu; Isobaric labeling; Mass spectrometry; Neuropeptides; Optimization.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Leucine / analogs & derivatives
  • Leucine / chemistry
  • Neuropeptides* / metabolism
  • Proteomics / methods
  • Tandem Mass Spectrometry* / methods

Substances

  • N,N-dimethyl leucine
  • Neuropeptides
  • Leucine