Mechanism of stable power generation and nitrogen removal in the ANAMMOX-MFC treating low C/N wastewater

Zhiyuan Kong; Yongheng Zhou; Zhimin Fu; Yuancan Zhang; Rong Yan

doi:10.1016/j.chemosphere.2022.133937

Mechanism of stable power generation and nitrogen removal in the ANAMMOX-MFC treating low C/N wastewater

Chemosphere. 2022 Jun:296:133937. doi: 10.1016/j.chemosphere.2022.133937. Epub 2022 Feb 12.

Authors

Zhiyuan Kong¹, Yongheng Zhou², Zhimin Fu³, Yuancan Zhang², Rong Yan²

Affiliations

¹ Key Laboratory of Environmental Pollution Control and Waste Resource Recycle, School of Ecology and Environment, Inner Mongolia University, Hohhot, 010031, China; Key Laboratory of Water and Sediment Sciences of Ministry of Education, State Key Laboratory of Water Environment Simulation, School of Environment, Beijing Normal University, Beijing, 100875, China.
² Key Laboratory of Environmental Pollution Control and Waste Resource Recycle, School of Ecology and Environment, Inner Mongolia University, Hohhot, 010031, China.
³ Key Laboratory of Environmental Pollution Control and Waste Resource Recycle, School of Ecology and Environment, Inner Mongolia University, Hohhot, 010031, China. Electronic address: fuzhm2012@163.com.

PMID: 35167835
DOI: 10.1016/j.chemosphere.2022.133937

Abstract

This study investigated the mechanism of enhanced power generation and nitrogen removal in an ANAMMOX-MFC reactor through subsequent acetate addition. Data showed that nearly 99% total nitrogen removal (≤1 mg L^-1) and 1.41 W m^-3 power generation were achieved synchronously under low COD/N (∼1.5) after the subsequent addition of acetate (100 mgCOD·L^-1). The columbic efficiency of the system has increased by 15 times (from 0.64% to 9.48%) after adding acetate. Batch tests showed that the denitrification and ANAMMOX progress occurred synchronously before acetate addition the nitrogen removal rate was accelerated. A distinct shift of bacterial community driven by acetate addition was discovered. The high throughput sequencing analysis indicated acetate addition stimulated the enrichment of denitrifiers, such as Aquimonas, Bradyrhizobium, Thauera, and the potential exoelectrogens changing from Comamonas to Pseudomonas. Functional genes forecasts based on KEGG database and COG database showed that the expressions of TCA functional genes were highly promoted in ANAMMOX-MFC, which demonstrated the enhanced electron transfer pathway driven by acetate addition under low COD/N ratio.

Keywords: ANAMMOX-MFC; Activated sludge in-situ hydrolysis-acidification; Microbial community; Subsequent carbon addition.

MeSH terms

Anaerobic Ammonia Oxidation
Bioreactors
Denitrification
Nitrogen*
Oxidation-Reduction
Sewage
Wastewater*

Substances

Sewage
Waste Water
Nitrogen