Homologous Recombination-Enhancing Factors Identified by Comparative Transcriptomic Analyses of Pluripotent Stem Cell of Human and Common Marmoset

Sho Yoshimatsu; Mayutaka Nakajima; Emi Qian; Tsukasa Sanosaka; Tsukika Sato; Hideyuki Okano

doi:10.3390/cells11030360

Homologous Recombination-Enhancing Factors Identified by Comparative Transcriptomic Analyses of Pluripotent Stem Cell of Human and Common Marmoset

Cells. 2022 Jan 21;11(3):360. doi: 10.3390/cells11030360.

Authors

Sho Yoshimatsu^{1

2}, Mayutaka Nakajima¹, Emi Qian¹, Tsukasa Sanosaka¹, Tsukika Sato¹, Hideyuki Okano^{1

2}

Affiliations

¹ Department of Physiology, Keio University School of Medicine, Tokyo 160-8582, Japan.
² Laboratory for Marmoset Neural Architecture, RIKEN Center for Brain Science, Saitama 351-0198, Japan.

Abstract

A previous study assessing the efficiency of the genome editing technology CRISPR-Cas9 for knock-in gene targeting in common marmoset (marmoset; Callithrix jacchus) embryonic stem cells (ESCs) unexpectedly identified innately enhanced homologous recombination activity in marmoset ESCs. Here, we compared gene expression in marmoset and human pluripotent stem cells using transcriptomic and quantitative PCR analyses and found that five HR-related genes (BRCA1, BRCA2, RAD51C, RAD51D, and RAD51) were upregulated in marmoset cells. A total of four of these upregulated genes enhanced HR efficiency with CRISPR-Cas9 in human pluripotent stem cells. Thus, the present study provides a novel insight into species-specific mechanisms for the choice of DNA repair pathways.

Keywords: common marmoset; homologous recombination; pluripotent stem cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Callithrix*
Embryonic Stem Cells / metabolism
Gene Editing
Homologous Recombination
Humans
Transcriptome* / genetics

Abstract

Publication types

MeSH terms

Grants and funding