Ultrasensitive detection of phenolphthalein in slimming products by gold-based immunochromatographic paper

Xiaoling Li; Xinxin Xu; Aihong Wu; Shanshan Song; Hua Kuang; Liqiang Liu; Zhengyou Wang; Chuanlai Xu

doi:10.1016/j.jpba.2022.114609

Ultrasensitive detection of phenolphthalein in slimming products by gold-based immunochromatographic paper

J Pharm Biomed Anal. 2022 Apr 1:212:114609. doi: 10.1016/j.jpba.2022.114609. Epub 2022 Jan 22.

Authors

Xiaoling Li¹, Xinxin Xu¹, Aihong Wu¹, Shanshan Song¹, Hua Kuang¹, Liqiang Liu², Zhengyou Wang³, Chuanlai Xu⁴

Affiliations

¹ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.
² State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China. Electronic address: murel@163.com.
³ Standards & Quality Center of National Food and Strategic Reserves Administration, Xicheng District, 100037 Beijing, People's Republic of China.
⁴ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China. Electronic address: xcl@jiangnan.edu.cn.

PMID: 35158187
DOI: 10.1016/j.jpba.2022.114609

Abstract

An anti-phenolphthalein monoclonal antibody (mAb) was prepared based on the N,N'-Carbonyldiimidazole (CDI) method through phenolphthalein conjugated with proteins. Indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and colloidal gold-based immunochromatographic assay (ICA) methods were used to determine phenolphthalein in slimming products. A standard curve was established, and the IC₅₀ and limit of detection of ic-ELISA were 0.95 and 0.10 ng/mL with a linear detection range of 0.27-3.37 ng/mL. The developed ICA was used to detect phenolphthalein in tablets, capsules, and slimming tea samples with visual limit of detection values of 10 μg/kg, and cut-off values of 200 μg/kg. The results indicated that these two methods could be used to quickly detect phenolphthalein in slimming products.

Keywords: Colloidal-gold immunochromatographic assay; Monoclonal antibody; Phenolphthalein; ic-ELISA.

MeSH terms

Chromatography, Affinity
Enzyme-Linked Immunosorbent Assay / methods
Gold Colloid*
Immunoassay / methods
Phenolphthalein*

Substances

Gold Colloid
Phenolphthalein