Nanobody-Based Assays for the Detection of Environmental and Agricultural Contaminants

Feng Wang; Hong Wang

doi:10.1007/978-1-0716-2075-5_28

Nanobody-Based Assays for the Detection of Environmental and Agricultural Contaminants

Methods Mol Biol. 2022:2446:547-554. doi: 10.1007/978-1-0716-2075-5_28.

Authors

Feng Wang¹, Hong Wang²

Affiliations

¹ College of Food Science, South China Agricultural University, Guangzhou, China.
² College of Food Science, South China Agricultural University, Guangzhou, China. gzwhongd@163.com.

PMID: 35157293
DOI: 10.1007/978-1-0716-2075-5_28

Abstract

Compared with traditional polyclonal and monoclonal antibodies, nanobodies derived from camelid heavy-chain antibodies have several advantages including small size, unique structure and binding geometry, high stability, and robust expression yields in numerous systems. Nanobody-based assays can also exhibit superior performance for immunodetection. Here, we describe protocols for three nanobody-based immunoassays for the detection of small chemical contaminants in environmental or agricultural samples: enzyme-linked immunosorbent assay (ELISA), fluorescence enzyme immunoassay (FEIA), and bioluminescent enzyme immunoassay (BLEIA). These methods are based on hapten-specific nanobodies, nanobody-alkaline phosphatase fusion proteins, and nanobody-nanoluciferase fusion proteins, respectively.

Keywords: Chemical contaminants; Fusion protein; Hapten; Immunodetection; Nanobody.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Enzyme-Linked Immunosorbent Assay / methods
Haptens
Immunoassay / methods
Immunoenzyme Techniques
Single-Domain Antibodies* / chemistry

Substances

Haptens
Single-Domain Antibodies