Creation of Multimeric Single-Domain Antibodies Using Bacterial Superglues

Paul J Wichgers Schreur; Sandra van de Water; Jeroen Kortekaas

doi:10.1007/978-1-0716-2075-5_15

Creation of Multimeric Single-Domain Antibodies Using Bacterial Superglues

Methods Mol Biol. 2022:2446:313-326. doi: 10.1007/978-1-0716-2075-5_15.

Authors

Paul J Wichgers Schreur¹, Sandra van de Water², Jeroen Kortekaas^{2

3}

Affiliations

¹ Department of Virology and Molecular Biology, Wageningen Bioveterinary Research, Lelystad, The Netherlands. paul.wichgersschreur@wur.nl.
² Department of Virology and Molecular Biology, Wageningen Bioveterinary Research, Lelystad, The Netherlands.
³ Laboratory of Virology, Wageningen University, Lelystad, The Netherlands.

PMID: 35157280
DOI: 10.1007/978-1-0716-2075-5_15

Abstract

Multimerization of single-domain antibodies (sdAbs) is instrumental for construction of antibody molecules with high avidity, extended in vivo half-life, and tailor-made biological activity. Two-component superglues, based on bacterium-derived peptides (Tags) and small protein domains (Catchers) that form isopeptide bonds when in close proximity, enable the creation of multimers by simply mixing of the individual components. Here, we provide detailed methods for the construction of sdAbs and scaffolds bearing genetically fused superglue components and their assembly into multimeric complexes.

Keywords: Multimers; Single-domain antibody; SnoopTag:SnoopCatcher; SpyTag:SpyCatcher; Superglue; VHH.

MeSH terms

Bacteria / genetics
Peptides / chemistry
Protein Domains
Single-Domain Antibodies* / genetics

Substances

Peptides
Single-Domain Antibodies