LINC00630 promotes cholangiocarcinoma cell proliferation, migration and invasion by mediating the miR-199a/FGF7 axis

Baiyin Zhong; Caixin Song; Qingfang He; Zhixi Chen; Qicheng Liao; Qiusheng Xiong; Shijie Wang; Yuansheng Xiao; Xing Xie; Yuankang Xie; Xiaonong Wang; Jianhong Zhang

doi:10.7150/jca.66850

LINC00630 promotes cholangiocarcinoma cell proliferation, migration and invasion by mediating the miR-199a/FGF7 axis

J Cancer. 2022 Jan 4;13(3):975-986. doi: 10.7150/jca.66850. eCollection 2022.

Authors

Affiliations

¹ Department of Hepatobiliary Surgery, the First Affiliated Hospital of Gannan Medical University, Ganzhou China, 341000.
² Department of General Surgery, the First Affiliated Hospital of Gannan Medical University, Ganzhou China, 341000.
³ College of Pharmacy, Gannan Medical University, Ganzhou China, 341000.

Abstract

Cholangiocarcinoma (CCA) is a type of cancer with a relatively low morbidity, but poor prognosis. Aberrant long non-coding RNA (lncRNA) expression has been observed in the pathological development of CCA. In the present study, lncRNA long intergenic non-protein coding RNA 630 (LINC00630) was found to be significantly upregulated in CCA tissues and cultured cells. LINC00630 expression was positively associated with histological differentiation, TNM stage and lymph node invasion. Short hairpin RNA (sh)-LINC00630 transfection could effectively decrease CCA cell proliferation, migration and invasion. Further investigations found that LINC00630 could interact with microRNA (miR)-199a, which specifically targeted fibroblast growth factor 7 (FGF7) for degradation. FGF7 overexpression restored the sh-LINC00630 transfection-induced decrease in CCA cell proliferation, migration and invasion. In conclusion, LINC00630 significantly promoted CCA cell proliferation, migration and invasion by upregulating FGF7 through miR-199a sponging.

Keywords: cholangiocarcinoma; fibroblast growth factor 7; invasion; long intergenic non-protein coding RNA 630; microRNA-199a; migration.