Morphological and Developmental Features of Stone Cells in Eriobotrya Fruits

Shoukai Lin; Dahe Lin; Bisha Wu; Shiwei Ma; Shengfeng Sun; Ting Zhang; Wenting Zhang; Yunlu Bai; Qiong Wang; Jincheng Wu

doi:10.3389/fpls.2022.823993

Morphological and Developmental Features of Stone Cells in Eriobotrya Fruits

Front Plant Sci. 2022 Jan 27:13:823993. doi: 10.3389/fpls.2022.823993. eCollection 2022.

Authors

Shoukai Lin^{1

2}, Dahe Lin^{1

2}, Bisha Wu^{1

2}, Shiwei Ma^{1

2}, Shengfeng Sun¹, Ting Zhang¹, Wenting Zhang¹, Yunlu Bai³, Qiong Wang³, Jincheng Wu^{1

2}

Affiliations

¹ College of Environmental and Biological Engineering, Putian University, Putian, China.
² Fujian Provincial Key Laboratory of Ecology-Toxicological Effects & Control for Emerging Contaminants, Putian University, Putian, China.
³ College of Horticulture, South China Agricultural University, Guangzhou, China.

Abstract

Some members of the Rosaceae family, particularly pear, contain stone cells in their fruits. Although stone cells in pear fruits are well studied, relatively little attention has been given to loquat stone cells. Only a few reports have suggested a relationship between stone cell traits and storage and transport tolerance of loquat fruits. Previously, we generated the variety JT8 from the interspecific hybrid of the loquat cultivar Jiefangzhong (JFZ; Eriobotrya japonica Lindl. cv. Jiefangzhong, female parent) and wild Taiwanese loquat (TL; E. deflexa Nakai, male parent). The JT8 fruits had a granular feel, similar to that of pear fruits, due to the presence of stone cells. In this study, the shape, size, development, and distribution dynamics of stone cells of Eriobotrya plants were thoroughly investigated. The results showed that loquat stone cells are brachysclereids and often contain typical branching pits. Loquat stone cells were distributed as both single stone cells and in stone cell clusters (SCCs), and the density of the stone cells near the core was higher than that near the peel. Stone cell density first increased and then decreased during fruit development. These traits noted in Eriobotrya were very similar to those observed in pear, indicating a close relationship between loquat and pear. Moreover, the contents, density dynamics, and aggregation traits of stone cells of the interspecific hybrid JT8 were derived from the male parent (TL). Transgressive segregation was likely exhibited in the content of stone cells and the size of the SCCs. More specifically, the content of stone cells reached 1.61% (w/w). In extreme cases, SCCs of JT8 exceeded 1,000 μm in length and 500 μm in width. This demonstrated that stone cell traits could be transmitted from parent to progeny through interspecific hybridization. The density dynamics of stone cells in two loquat cultivars with different storage and transport tolerances were also investigated, which indicated that the cultivar with more stone cells was more tolerant to storage and transport. We suggest that wild loquat genetic resources containing stone cells in Eriobotrya plants can be used to gradually improve the storage and transport tolerance of loquat fruits.

Keywords: Eriobotrya plants; fruits; morphological and developmental features; stone cells; trait transmission.