Removal of Escherichia coli from domestic sewage using biological sand filters: Reduction effect and microbial community analysis

Abstract

The recycling of sewage is an economical option to solve the water resource pressure. However, to avoid health risks to humans, pathogens in sewage must be removed before reuse. In this study, a biological sand filter (BSF) was used to remove pathogen indicator Escherichia coli (E. coli) from sewage. The biolayer (schmutzdecke layer) formation process of BSFs, operation performance, factors affecting E. coli removal and microbial community structure were evaluated. The results of schmutzdecke layer culture showed that a large number of microorganisms were attached to the upper medium of BSFs. At the same time, the BSFs could reduce both conventional contaminants and E. coli. The E. coli removal experiments revealed that the removal rate of E. coli was about 96.1% at higher effective medium depth (50 cm), the removal rate was about 95% when set hydraulic loading rate (HLR) to 0.16 m³/m²/h and the removal efficiency reached 93.6% at lower influent bacteria concentration. Finally, the microbial community analysis indicated that different BSFs had similar microbial structure, and the microbial abundance in the schmutzdecke layer was higher than that in the bottom layer in the same BSFs. Besides, Biological action played a significant role in the removal of E. coli, including the bacteriolysis of Bdellovibrio and the competition between other bacteria and E. coli. In summary, BSF was a promising technology for removing E. coli from sewage.

Keywords: Biological sand filter; Escherichia coli removal; Microbial community; Schmutzdecke layer.