Methods to Investigate Cell Polarity of Inner Ear

Jihan Lyu; Xiaoqing Qian; Binjun Chen; Dongdong Ren

doi:10.1007/978-1-0716-2035-9_16

Methods to Investigate Cell Polarity of Inner Ear

Methods Mol Biol. 2022:2438:251-275. doi: 10.1007/978-1-0716-2035-9_16.

Authors

Jihan Lyu^{1

2}, Xiaoqing Qian^{1

2}, Binjun Chen^{1

2}, Dongdong Ren^{3

4}

Affiliations

¹ ENT institute and Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, China.
² NHC Key Laboratory of Hearing Medicine (Fudan University), Shanghai, China.
³ ENT institute and Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, China. dongdongren@fudan.edu.cn.
⁴ NHC Key Laboratory of Hearing Medicine (Fudan University), Shanghai, China. dongdongren@fudan.edu.cn.

PMID: 35147947
DOI: 10.1007/978-1-0716-2035-9_16

Abstract

Hair cells in cochlea and vestibular organs depend on the coordinated cell polarity to perform the normal auditory or balance function. The mouse inner ear is one of the ideal model to study planar cell polarity. In this chapter, we introduce a series of general experimental methods for studying planar cell polarity in the inner ear. The approaches presented here are also applicable to other organs with particular polarity phenotypes.

Keywords: Cochlea; Culture; Hair cell polarity; Inner ear dissection; Orientation of stereocilia bundles; Paint filling; SEM; Saccule; Utricle.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Polarity*
Cochlea
Hair Cells, Auditory
Mice
Vestibule, Labyrinth*