Electrochemical sensing of Staphylococcus aureus based on conductive anti-fouling interface

Huanli Gao; Tingting Xu; Jiahuan Zhou; Orlando J Rojas; Ming He; Xingxiang Ji; Hongqi Dai

doi:10.1007/s00604-022-05190-z

Electrochemical sensing of Staphylococcus aureus based on conductive anti-fouling interface

Mikrochim Acta. 2022 Feb 11;189(3):97. doi: 10.1007/s00604-022-05190-z.

Authors

Huanli Gao¹, Tingting Xu^{2

3

4}, Jiahuan Zhou¹, Orlando J Rojas⁵, Ming He⁶, Xingxiang Ji⁶, Hongqi Dai⁷

Affiliations

¹ Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, International Innovation Center for Forest Chemicals and Materials, College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing, 210037, China.
² Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, International Innovation Center for Forest Chemicals and Materials, College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing, 210037, China. salyting@njfu.edu.cn.
³ Bioproducts Institute, Department of Chemical and Biological Engineering, Department of Chemistry and Department of Wood Science, The University of British Columbia, 2360 East Mall, Vancouver, BC, V6T 1Z3, Canada. salyting@njfu.edu.cn.
⁴ State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan, 250353, China. salyting@njfu.edu.cn.
⁵ Bioproducts Institute, Department of Chemical and Biological Engineering, Department of Chemistry and Department of Wood Science, The University of British Columbia, 2360 East Mall, Vancouver, BC, V6T 1Z3, Canada.
⁶ State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan, 250353, China.
⁷ Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, International Innovation Center for Forest Chemicals and Materials, College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing, 210037, China. hgdhq@njfu.edu.cn.

PMID: 35147807
DOI: 10.1007/s00604-022-05190-z

Abstract

A system for the rapid and ultra-sensitive detection of Staphylococcus aureus (S. aureus), a prevalent foodborne pathogen is introduced. Limitations of typical electrochemical sensing, often subjected to interference from non-specific protein adsorption are addressed. A dual-aptamer-based sandwich immunobiosensor is shown for its benefits regarding specificity and anti-fouling capacity, endowed by a sulfonated polyaniline layer combined with signal amplification via highly conductive gold nanoparticles. EIS spectra (Nyquist plots) were recorded at pH 7.4 PBS containing 5 mM Fe(CN)₆^3-/Fe(CN)₆^4-, in order to verify the possibility of the electrochemical sensing for detection of S. aureus. Results demonstrated that the constructed immunobiosensor presents an extended detection range (1 × 10¹ to 1 × 10⁵ CFU/mL) and detection limit as low as 2 CFU/mL. The resistance values of the immunobiosensor developed maintain at a stable value during 2 weeks. Besides, the specificity of the system is highlighted by testing raw milk, and the results of which demonstrate the excellent prospects of the system for monitoring foodborne pathogens.

Keywords: Anti-fouling; Dual-aptamer sensor; Sandwich immunobiosensors; Staphylococcus aureus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aptamers, Nucleotide / chemistry
Biofouling
Biosensing Techniques*
Electrochemical Techniques*
Food Contamination / analysis*
Staphylococcus aureus / isolation & purification*

Substances

Aptamers, Nucleotide

Abstract

Publication types

MeSH terms

Substances

Grants and funding