The Group 3 LEA proteins of Artemia franciscana for cryopreservation

Lu Li; Xuexun Zhou; Zhongrong Chen; Yunxia Cao; Gang Zhao

doi:10.1016/j.cryobiol.2022.01.004

The Group 3 LEA proteins of Artemia franciscana for cryopreservation

Cryobiology. 2022 Jun:106:1-12. doi: 10.1016/j.cryobiol.2022.01.004. Epub 2022 Feb 4.

Authors

Lu Li¹, Xuexun Zhou², Zhongrong Chen³, Yunxia Cao⁴, Gang Zhao⁵

Affiliations

¹ School of Basic Medicine, Anhui Medical University, Hefei, 230032, PR China.
² ICryobio Technologies, Shanghai, 201403, PR China.
³ School of Biomedical Engineering, Research and Engineering Center of Biomedical Materials, Anhui Medical University, Hefei, 230032, PR China. Electronic address: zhrchen@ahmu.edu.cn.
⁴ Department of Obstetrics and Gynecology, Reproductive Medicine Center, The First Affiliated Hospital of Anhui Medical University, Hefei, 230032, PR China; NHC Key Laboratory of Study on Abnormal Gametes and Reproductive Tract (Anhui Medical University), Hefei, 230032, PR China. Electronic address: caoyunxia6@126.com.
⁵ School of Biomedical Engineering, Research and Engineering Center of Biomedical Materials, Anhui Medical University, Hefei, 230032, PR China; Department of Electronic Science and Technology, University of Science and Technology of China, Hefei, 230027, PR China. Electronic address: zhaog@ustc.edu.cn.

PMID: 35131291
DOI: 10.1016/j.cryobiol.2022.01.004

Abstract

Late embryogenesis-abundant (LEA) proteins are protective proteins that are enriched in the late stage of seed embryo development. LEA proteins play an important role in resisting abiotic stresses such as low temperature and drought. Artemia franciscana is the only animal known to express three different groups of LEA proteins in its life cycle, and the discovery has some applications for the cryopreservation of human cells. In this review, A. franciscana LEA proteins from Group 3 are systematically introduced, and the structure, location, function and application in cryopreservation are highlighted. As a nontoxic and effective cryoprotectant, A. franciscana LEA proteins are expected to provide a new method for cryopreservation of cells.

Keywords: Cryopreservation; Function; Late embryogenesis-abundant proteins; Location; Structure.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Artemia* / metabolism
Cryopreservation* / methods
Cryoprotective Agents / metabolism
Cryoprotective Agents / pharmacology
Embryonic Development
Plant Proteins / metabolism
Proteins / metabolism

Substances

Cryoprotective Agents
Plant Proteins
Proteins