Sustained release of naringin from silk-fibroin-nanohydroxyapatite scaffold for the enhancement of bone regeneration

Zhi-Hu Zhao; Xin-Long Ma; Jian-Xiong Ma; Jia-Yu Kang; Yang Zhang; Yue Guo

doi:10.1016/j.mtbio.2022.100206

Sustained release of naringin from silk-fibroin-nanohydroxyapatite scaffold for the enhancement of bone regeneration

Mater Today Bio. 2022 Jan 23:13:100206. doi: 10.1016/j.mtbio.2022.100206. eCollection 2022 Jan.

Authors

Zhi-Hu Zhao¹, Xin-Long Ma¹, Jian-Xiong Ma², Jia-Yu Kang³, Yang Zhang², Yue Guo²

Affiliations

¹ Department of Orthopaedics, Tianjin Hospital, No. 406, Jiefangnan Road, Hexi District, Tianjin, 300000, China.
² Tianjin Institute of Orthopedics in Traditional Chinese and Western Medicine, No. 122, Munan Road, Tianjin, 300050, China.
³ Department of Orthopedics, Jinhua Municipal Central Hospital, Jinhua, Zhejiang Province, China.

Abstract

Bone defects are a common challenge in the clinical setting. Bone tissue engineering (BTE) is an effective treatment for the clinical problem of large bone defects. In this study, we fabricated silk fibroin (SF)/hydroxyapatite (HAp) scaffolds inlaid with naringin poly lactic-co-glycolic acid (PLGA) microspheres, investigating the feasibility of their application in BTE. Naringin PLGA microspheres were manufactured and adhered to the SF/HAp scaffold. Bone mesenchymal stem cells (BMSCs) were inoculated onto the SF/HAp scaffold containing naringin PLGA microsphere to examine the biocompatibility of the SF/HAp scaffolds. A rabbit femoral distal bone defect model was used to evaluate the in vivo function of the SF/HAp scaffolds containing naringin-loaded PLGA microspheres. The current study demonstrated that SF/HAp scaffolds containing naringin-loaded PLGA microspheres show promise as osteo-modulatory biomaterials for bone regeneration.

Keywords: ALP, Alkaline phosphatase activity; ANOVA, one-way analysis of variance; BMSCs, Bone mesenchymal stem cells; BP, biological process; BTE, Bone tissue engineering; Bone defect; CC, cellular component; CCK-8, Cell count kit-8; DAVID, database for annotation, visualization, and integrated discovery; GO, Gene ontology; HAp, hydroxyapatite; HUVEC, human umbilical endothelial cells; Hydroxyapatite; KEGG, Kyoto Encyclopedia of Genes and Genomes; MF, molecular function; Microsphere; Naringin; PLGA; PLGA, poly lactic-co-glycolic acid; PVA, Polyvinyl alcohol; RNA-Seq, RNA sequencing; RT-PCR, real-time quantitative polymerase chain reaction; SEM, scanning electron microscopy; SF, silk fibroin; Silk.