Fractionation and Extraction of Crude Nuclear Proteins From Arabidopsis Seedlings

Jiajia Zhao; Jinsong Bao; Feifei Xu

doi:10.21769/BioProtoc.4296

Fractionation and Extraction of Crude Nuclear Proteins From Arabidopsis Seedlings

Bio Protoc. 2022 Jan 20;12(2):e4296. doi: 10.21769/BioProtoc.4296.

Authors

Jiajia Zhao¹, Jinsong Bao¹, Feifei Xu¹

Affiliation

¹ Institute of Nuclear Agricultural Sciences, Zhejiang University, Zijingang Campus, Hangzhou 310058, China.

Abstract

The plant nucleus is an important subcellular organelle that contains the genome, ribosomal RNA, and regulatory proteins, and performs a central role in the functioning and metabolism of the cell. Fractionation of intact nuclei is a crucial process to elucidate the function of nuclear proteins. Here, we present a simple method for the fractionation of crude nuclei and extraction of nuclear proteins, based on previously established methods. This protocol provides an easy and quick method to isolate crude nuclei and extract nuclear proteins from Arabidopsis seedlings, which is useful for the research on the nuclear proteins, without requirement for high-purity nuclei. Graphic abstract: Schematic procedure for the isolation of crude nuclei and extraction of nuclear proteins from Arabidopsis seedlings.

Keywords: Arabidopsis seedlings; Crude nuclei isolation; Nuclear protein extraction; Protocol.