Diversity and structure of the microbial community in rhizosphere soil of Fritillaria ussuriensis at different health levels

Ning Jiao; Xiaoshuang Song; Ruiqing Song; Dachuan Yin; Xun Deng

doi:10.7717/peerj.12778

Diversity and structure of the microbial community in rhizosphere soil of Fritillaria ussuriensis at different health levels

PeerJ. 2022 Jan 25:10:e12778. doi: 10.7717/peerj.12778. eCollection 2022.

Authors

Ning Jiao¹, Xiaoshuang Song², Ruiqing Song¹, Dachuan Yin³, Xun Deng²

Affiliations

¹ College of Forestry, Northeast Forestry University, Harbin, China.
² Institute of Forestry Protection, Heilongjiang Forestry Academy, Harbin, China.
³ College of Forestry, Shenyang Agricultural University, Shenyang, China.

Abstract

Fritillaria wilt is a kind of soil-borne disease that causes a large reduction in the yield of Fritillaria ussuriensis. The diversity and structure of the soil microbial community are important factors affecting the health of Fritillaria ussuriensis. The analysis of the microbial community in the diseased and healthy soils provided a theoretical basis for revealing the pathological mechanism and prevention of Fritillaria wilt disease. In the present study, we sequenced the soil microorganisms from healthy (H), pathology (P) and blank (B) soil samples by Illumina MiSeq. Determined the soil physicochemical properties respectively, analyzed the soil microbial diversity and structure, and constructed single factor co-correlation networks among microbial genera. The results showed that Ascomycota (48.36%), Mortierellomycota (23.06%), Basidiomycota (19.00%), Proteobacteria (31.74%), and Acidobacteria (20.95%) were dominant in the soil. The diversity of healthy soil was significantly greater than that of diseased soil samples (P and B) (P < 0.05). The populations of Fusarium and Humicola significantly increased in the diseased soil sample (P and B) (P < 0.05). RB41 (4.74%) and Arthrobacter (3.30%) were the most abundant genera in the healthy soil. Total nitrogen (TN), available nitrogen (AN), total potassium (TK), available potassium (AK), and inorganic salt (salt) were significantly correlated with soil microbial communities (P < 0.05). The relationship between fungi and the plant was mostly positive, whereas bacteria showed the opposite trend. In conclusion, the diversity and structure of the soil microbial community were closely related to the health level of Fritillaria ussuriensis. Fusarium and Humicola affect the severity of Fritillaria wilt disease, while RB41 and Arthrobacter are the important indicators for maintaining the health of Fritillaria ussuriensis. Moreover, environmental factors greatly affect the abundance and formation of soil microbial community. The interactions in microbial communities also influence the healthy growth of Fritillaria ussuriensis.

Keywords: Health level; Illumina MiSeq; Soil microbial community; Fritillaria wilt disease.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Ascomycota*
Fritillaria*
Fusarium*
Health Status
Microbiota* / genetics
Rhizosphere
Soil / chemistry

Substances

Soil

Grants and funding

This research was funded by (National Key Research and Development Program) grant number (2016YFC0500308-08), (National Natural Science Foundation of China) grant number (31670649, 31700564, 31170597,31200484). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.