Target-induced silver nanocluster generation for highly sensitive electrochemical aptasensor towards cell-secreted interferon-γ

Yanli Zhou; Jiaxiang Liu; Hui Dong; Zi Liu; Lu Wang; Qiaoxia Li; Jianhua Ren; Yintang Zhang; Maotian Xu

doi:10.1016/j.bios.2022.114042

Target-induced silver nanocluster generation for highly sensitive electrochemical aptasensor towards cell-secreted interferon-γ

Biosens Bioelectron. 2022 May 1:203:114042. doi: 10.1016/j.bios.2022.114042. Epub 2022 Jan 31.

Authors

Yanli Zhou¹, Jiaxiang Liu², Hui Dong³, Zi Liu³, Lu Wang³, Qiaoxia Li⁴, Jianhua Ren², Yintang Zhang², Maotian Xu⁵

Affiliations

¹ Henan Key Laboratory of Biomolecular Recognition and Sensing, Henan Joint International Research Laboratory of Chemo/Biosensing and Early Diagnosis of Major Diseases, College of Chemistry and Chemical Engineering, Shangqiu Normal University, Shangqiu, 476000, PR China. Electronic address: zhouyanli@mails.ucas.ac.cn.
² College of Environmental and Chemical Engineering, Shanghai University of Electric Power, Shanghai, 20090, PR China.
³ Henan Key Laboratory of Biomolecular Recognition and Sensing, Henan Joint International Research Laboratory of Chemo/Biosensing and Early Diagnosis of Major Diseases, College of Chemistry and Chemical Engineering, Shangqiu Normal University, Shangqiu, 476000, PR China.
⁴ College of Environmental and Chemical Engineering, Shanghai University of Electric Power, Shanghai, 20090, PR China. Electronic address: liqiaoxia@shiep.edu.cn.
⁵ Henan Key Laboratory of Biomolecular Recognition and Sensing, Henan Joint International Research Laboratory of Chemo/Biosensing and Early Diagnosis of Major Diseases, College of Chemistry and Chemical Engineering, Shangqiu Normal University, Shangqiu, 476000, PR China. Electronic address: xumaotian@163.com.

PMID: 35124342
DOI: 10.1016/j.bios.2022.114042

Abstract

Interferon-gamma (IFN-γ) is one kind of crucial inflammatory cytokines, and its expression level is closely associated with various disease progressions. This work addresses the development of a sensitive and specific electrochemical assay for detection of IFN-γ by combing the recognition unit of aptamer with the signal reporter of target-induced silver nanoclusters (AgNCs). For biosensor preparation, the gold nanoparticles (AuNPs) immobilized on the amine-terminated electrode surface provided electrochemical interfaces for the self-assembly of C-rich modified aptamers. Then, the aptamer recognized IFN-γ and the free aptamer hybridized with conjugated DNA sequences. After the nuclease-catalyzed cleavage of DNA duplex, in situ-generated AgNCs in the C-rich template was utilized as the electrochemical indicator for IFN-γ detection. The present method demonstrated a good performance for detection of IFN-γ with a low detection limit of 1.7 pg mL^-1. This aptasensor was verified to be applied for the evaluation of IFN-γ secreted by cell.

Keywords: Aptasensor; C-rich template; Cell secretion; Electrochemical label; Interferon-gamma; Silver nanoclusters.

MeSH terms

Aptamers, Nucleotide* / metabolism
Biosensing Techniques* / methods
Electrochemical Techniques / methods
Gold
Interferon-gamma / metabolism
Limit of Detection
Metal Nanoparticles*
Silver

Substances

Aptamers, Nucleotide
Silver
Gold
Interferon-gamma