Elevated levels of IL-32 in cerebrospinal fluid of neuro-Behcet disease: Correlation with NLRP3 inflammasome

J Neuroimmunol. 2022 Apr 15:365:577820. doi: 10.1016/j.jneuroim.2022.577820. Epub 2022 Jan 30.

Abstract

Interleukin-32 (IL-32) is a pro-inflammatory cytokine that induces other cytokines involved in inflammation, including tumour necrosis factor (TNF)-α, IL-6 and IL-1β. The objective of this study was to evaluate IL-32, NLRP3 inflammasome, IL-1β, IL-6, IL-17A, TNF-a, IL-10 and IL-37 in cerebrospinal fluid (CSF) and paired serum samples of patients with neuro-Behcet disease (NBD) by ELISA, RT-PCR and Western blotting analysis. A receiver operating characteristic (ROC) curve was employed to explore of the predictive value of IL-32 levels. IL-32, IL-1β, IL-6, IL-17 and TNF-α, were highly expressed in CSF of NBD and multiple sclerosis (MS) patients contrasting with their low levels in patients with noninflammatory neurological diseases (NIND) and Headache attributed to BD (HaBD). IL-32 and NLRP3 inflammasome in NBD, correlate significantly with CRP and ESR. IL-32 should be studied further as potential BD biomarker of inflammation in NBD.

Keywords: CSF; IL-32; Inflammasome; NLRP3; Neuro-Behcet disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Behcet Syndrome* / cerebrospinal fluid
  • Cytokines / cerebrospinal fluid
  • Humans
  • Inflammasomes*
  • Inflammation / pathology
  • Interleukin-1beta
  • Interleukin-6
  • Interleukins
  • NLR Family, Pyrin Domain-Containing 3 Protein
  • Tumor Necrosis Factor-alpha

Substances

  • Cytokines
  • IL32 protein, human
  • Inflammasomes
  • Interleukin-1beta
  • Interleukin-6
  • Interleukins
  • NLR Family, Pyrin Domain-Containing 3 Protein
  • NLRP3 protein, human
  • Tumor Necrosis Factor-alpha