Evaluation of Innate Lymphoid Cells and Dendritic Cells Following Viral Vector Vaccination

Zheyi Li; Sreeja Roy; Charani Ranasinghe

doi:10.1007/978-1-0716-2168-4_8

Evaluation of Innate Lymphoid Cells and Dendritic Cells Following Viral Vector Vaccination

Methods Mol Biol. 2022:2465:137-153. doi: 10.1007/978-1-0716-2168-4_8.

Authors

Zheyi Li¹, Sreeja Roy^{1

2}, Charani Ranasinghe³

Affiliations

¹ Molecular Mucosal Vaccine Immunology Group, Division of Immunity, Inflammation and Infection, The John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia.
² Aging, Cancer and Immuno-Oncology , Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA, USA.
³ Molecular Mucosal Vaccine Immunology Group, Division of Immunity, Inflammation and Infection, The John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia. Charani.Ranasinghe@anu.edu.au.

PMID: 35118620
DOI: 10.1007/978-1-0716-2168-4_8

Abstract

Recently, we have shown that fate of a vaccine is determined by the cytokine milieu in the innate immune compartment at the early stage of vaccination. Specifically, 24 h post-delivery, level of innate lymphoid cell type 2 (ILC2)-derived IL-13/IL-13Rα2 are the master regulators of DC and also different ILC subsets responsible for modulating the downstream immune outcomes. Here, we provide step-by-step details how to assess different ILC and DC subsets in lung and muscle following intranasal and intramuscular viral vector vaccination, respectively, using multi-color flow cytometry and confocal microscopy.

Keywords: Confocal microscopy; Dendritic cells (DC); Flow cytometry; IL-4/IL-13 regulation; Innate lymphoid cells (ILC); Intranasal (i.n.)/intramuscular (i.m.) vaccination; Lung and muscle single cell preparation; Recombinant fowl poxvirus (rFPV); Viral vector vaccination.

MeSH terms

Dendritic Cells
Immunity, Innate*
Lymphocytes
Vaccination
Viral Vaccines*

Substances

Viral Vaccines