Production and Purification of Candidate Subunit Vaccines by IC-Tagging Protein Encapsulation

Natalia Barreiro-Piñeiro; Tomás Pose-Boirazian; Rebeca Menaya-Vargas; José M Martínez-Costas

doi:10.1007/978-1-0716-2168-4_2

Production and Purification of Candidate Subunit Vaccines by IC-Tagging Protein Encapsulation

Methods Mol Biol. 2022:2465:27-40. doi: 10.1007/978-1-0716-2168-4_2.

Authors

Natalia Barreiro-Piñeiro¹, Tomás Pose-Boirazian¹, Rebeca Menaya-Vargas¹, José M Martínez-Costas²

Affiliations

¹ Department of Biochemistry and Molecular Biology, Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CIQUS), University of Santiago de Compostela, A Coruña, Spain.
² Department of Biochemistry and Molecular Biology, Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CIQUS), University of Santiago de Compostela, A Coruña, Spain. jose.martinez.costas@usc.es.

PMID: 35118614
DOI: 10.1007/978-1-0716-2168-4_2

Abstract

Particulate material is more efficient in eliciting immune responses. Here we describe the production of micro- and nanospheres formed by protein muNS-Mi from avian reoviruses, loaded with foreign epitopes for their use as vaccines.

Keywords: Adjuvants; Avian reovirus; IC-Tagging; Microspheres; Nanospheres; Particulate material; Vaccines.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adjuvants, Immunologic
Epitopes / metabolism
Orthoreovirus, Avian* / metabolism
Vaccines* / metabolism
Vaccines, Subunit
Viral Nonstructural Proteins / metabolism

Substances

Adjuvants, Immunologic
Epitopes
Vaccines
Vaccines, Subunit
Viral Nonstructural Proteins