A protocol for quantifying lymphocyte-mediated cytotoxicity using an impedance-based real-time cell analyzer

Hisashi Kanemaru; Yukari Mizukami; Akira Kaneko; Ikko Kajihara; Satoshi Fukushima

doi:10.1016/j.xpro.2022.101128

A protocol for quantifying lymphocyte-mediated cytotoxicity using an impedance-based real-time cell analyzer

STAR Protoc. 2022 Jan 22;3(1):101128. doi: 10.1016/j.xpro.2022.101128. eCollection 2022 Mar 18.

Authors

Hisashi Kanemaru¹, Yukari Mizukami¹, Akira Kaneko¹, Ikko Kajihara¹, Satoshi Fukushima¹

Affiliation

¹ Department of Dermatology and Plastic Surgery, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjo, Kumamoto 860-8556, Japan.

Abstract

Current standard assays to analyze lymphocyte-mediated antitumor cytotoxicity employ radioisotopic or fluorescent labels. However, such assays are not suitable for real-time analysis. Here we describe a protocol that facilitates the analysis of lymphocyte-mediated toxicity using a label-free, impedance-based real-time cell analyzer. This analyzer measures cellular electrical impedance, expressed as the cell index value, noninvasively and continuously. In contrast with label-dependent assays, this protocol simultaneously generates real-time killing curves useful for quantifying lymphocyte-mediated cytotoxicity in real time. For complete details on the use and execution of this protocol, please refer to Kanemaru et al. (2021).

Keywords: Cancer; Cell Biology; Immunology.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biological Assay*
Cytotoxicity, Immunologic*
Electric Impedance