Identification of heavy metals tolerant Brevundimonas sp. from rhizospheric zone of Saccharum munja L. and their efficacy in in-situ phytoremediation

Pooja Sharma; Preeti Chaturvedi; Ram Chandra; Sunil Kumar

doi:10.1016/j.chemosphere.2022.133823

Identification of heavy metals tolerant Brevundimonas sp. from rhizospheric zone of Saccharum munja L. and their efficacy in in-situ phytoremediation

Chemosphere. 2022 May:295:133823. doi: 10.1016/j.chemosphere.2022.133823. Epub 2022 Feb 1.

Authors

Pooja Sharma¹, Preeti Chaturvedi², Ram Chandra³, Sunil Kumar⁴

Affiliations

¹ Department of Environmental Microbiology, School for Environmental Sciences, Babasaheb Bhimrao Ambedkar (A Central) University, Vidya Vihar, Raebareli Road, Lucknow, Uttar Pradesh, 226 025, India; CSIR-National Environmental Engineering Research Institute (CSIR-NEERI), Nehru Marg, Nagpur, 440 020, Maharashtra, India. Electronic address: sharmaspspooja@gmail.com.
² Aquatic Toxicology Laboratory, Environmental Toxicology Group, Council of Scientific and Industrial Research-Indian Institute of Toxicology Research (CSIR-IITR), Vishvigyan Bhawan, 31, M.G. Marg, Lucknow, 226 001, Uttar Pradesh, India.
³ Department of Environmental Microbiology, School for Environmental Sciences, Babasaheb Bhimrao Ambedkar (A Central) University, Vidya Vihar, Raebareli Road, Lucknow, Uttar Pradesh, 226 025, India. Electronic address: Prof.chandrabbau@gmail.com.
⁴ CSIR-National Environmental Engineering Research Institute (CSIR-NEERI), Nehru Marg, Nagpur, 440 020, Maharashtra, India. Electronic address: s_kumar@neeri.res.in.

PMID: 35114263
DOI: 10.1016/j.chemosphere.2022.133823

Abstract

Heavy metals phytoremediation from pulp and paper industry (PPI) sludge was conducted by employing root-associated Brevundimonas sp (PS-4 MN238722.1) in rhizospheric zone of Saccharum munja L. for its detoxification. The study was aimed to investigate the efficiency of Saccharum munja L. for the removal of heavy metals along with physico-chemical parameters through bacterial interactions. Physico-chemical examination of PPI sludge showed biochemical oxygen demand (8357 ± 94 mg kg^-1), electrical conductivity (2264 ± 49 μmhoscm^-1), total phenol (521 ± 24 mg kg^-1), total dissolve solid (1547 ± 23 mg kg^-1), total nitrogen (264 ± 2.13 mg kg^-1), pH (8.2 ± 0.11), chemical oxygen demand (34756 ± 214 mg kg^-1), color (2434 ± 45 Co-Pt), total suspended solid (76 ± 0.67 mg kg^-1), sulphate (2462 ± 13 mg kg^-1), chlorolignin (597 ± 13.01 mg kg^-1), K⁺ (21.04 ± 0.26 mg kg^-1), total solid (1740 ± 54 mg kg^-1), phosphorous, Cl^-, and Na⁺. Heavy metals, such as Fe followed by Zn, Mn, Cd, Cu, Ni, Pb, As, Cr and Hg were above the permissible limit. Root and shoot of Saccharum munja L. revealed highest concentrations of Cd followed by Mn, Ni, Fe, Zn, Cu, As, Cr, Hg, and Pb. Tested metals (Fe, Mn, Pb, Cd, Cr, Cu, Zn, Ni, As, and Hg) bioaccumulation and translocation factors were also revealed to be < 1 and >1, respectively, demonstrating that these plants have considerable absorption and translocation abilities. Plant growth-promoting activity, such as ligninolytic enzymes, hydrolytic enzymes, indole acetic acid, and siderophore production activity of Brevundimonas sp. (PS-4 MN238722.1) were also noted to be higher. These findings support the use of Brevundimonas sp (PS-4 MN238722.1) in combination with Saccharum munja L. plant as interdisciplinary management of industrial sludge at polluted areas for the prevention of soils near the industrial site.

Keywords: Bacterial efficiency; Environmental protection; Phytoaccumulation; Sludge treatment; Toxic elements.

MeSH terms

Biodegradation, Environmental
Environmental Monitoring
Metals, Heavy* / analysis
Saccharum*
Soil
Soil Pollutants* / analysis

Substances

Metals, Heavy
Soil
Soil Pollutants