Fast automated method for the direct determination of total antimony in grape juice samples by hydride generation and atomic fluorescence spectrometric detection without external pretreatment

Eduardo A Lima; Francisco A S Cunha; Maria J Oliveira; Wellington S Lyra; Mario M S Junior; Josué C C Santos; Sergio L C Ferreira; Mario C U Araujo; Luciano F Almeida

doi:10.1016/j.foodchem.2022.132194

Fast automated method for the direct determination of total antimony in grape juice samples by hydride generation and atomic fluorescence spectrometric detection without external pretreatment

Food Chem. 2022 Jul 1:381:132194. doi: 10.1016/j.foodchem.2022.132194. Epub 2022 Jan 21.

Authors

Eduardo A Lima¹, Francisco A S Cunha², Maria J Oliveira², Wellington S Lyra¹, Mario M S Junior³, Josué C C Santos², Sergio L C Ferreira⁴, Mario C U Araujo¹, Luciano F Almeida⁵

Affiliations

¹ Universidade Federal da Paraíba, Departamento de Química, P.O. Box 5093, Zip Code 58051-970, João Pessoa, PB, Brazil.
² Universidade Federal de Alagoas, Instituto de Química e Biotecnologia, Campus A.C. Simões, Tabuleiro dos Martins, 57072-900, Maceió, AL, Brazil.
³ Universidade Federal do Sul da Bahia, s/n, BR 367, km 10, 45810-000, Porto Seguro, BA, Brazil.
⁴ Universidade Federal da Bahia, Instituto de Química, Campus Ondina, 40170-115 Salvador, BA, Brazil.
⁵ Universidade Federal da Paraíba, Departamento de Química, P.O. Box 5093, Zip Code 58051-970, João Pessoa, PB, Brazil. Electronic address: luciano@quimica.ufpb.br.

PMID: 35101706
DOI: 10.1016/j.foodchem.2022.132194

Abstract

Matrix complexity of fruit juices and their low antimony level requires sensitive, cost-effective instruments, time-consuming and error-prone sample pretreatment methods. Therefore, a flow-batch procedure (HG-FBA-AFS) was developed for the fast and sensitive determination of total inorganic Sb in grape juice samples by hydride generation atomic fluorescence spectrometry. The sample pretreatment, pre-reduction and stibine formation steps run through the mixing chamber. The HCl and NaBH₄ concentrations, and carrier gas flowrate were optimized through a Box-Behnken design. The detection limit (LOD) was 20 ng L^-1, intra and inter-day precision ranged in 3.0 - 3.5 %, and low errors within (- 2.4 to 6.6 %) for samples containing 1.23 - 4.58 µg L^-1 total Sb. Both HG-FBA-AFS and reference method agreed at 95% confidence level. An 87 h^-1 sample throughput, and a 1.15 mL total waste per determination attest that HG-FBA-AFS is a fast, and ecofriendly tool for determining Sb in grape juices.

Keywords: Box-Behnken design; Flow-batch analysis; Grape-fruit products; HG-AFS; Online sample preparation; Total inorganic antimony.

MeSH terms

Antimony* / analysis
Fruit and Vegetable Juices / analysis
Spectrometry, Fluorescence / methods
Spectrophotometry, Atomic
Vitis*

Substances

Antimony