Molecular phylogeny and taxonomy of the Hydrangeaserrata complex (Hydrangeaceae) in western Japan, including a new subspecies of H.acuminata from Yakushima

Shun K Hirota; Tetsukazu Yahara; Kengo Fuse; Hiroyuki Sato; Shuichiro Tagane; Shinji Fujii; Tadashi Minamitani; Yoshihisa Suyama

doi:10.3897/phytokeys.188.64259

Molecular phylogeny and taxonomy of the Hydrangeaserrata complex (Hydrangeaceae) in western Japan, including a new subspecies of H.acuminata from Yakushima

PhytoKeys. 2022 Jan 12:188:49-71. doi: 10.3897/phytokeys.188.64259. eCollection 2022.

Authors

Shun K Hirota¹, Tetsukazu Yahara², Kengo Fuse², Hiroyuki Sato², Shuichiro Tagane³, Shinji Fujii⁴, Tadashi Minamitani⁵, Yoshihisa Suyama¹

Affiliations

¹ Field Science Center, Graduate School of Agricultural Science, Tohoku University, 232-3 Yomogida, Naruko-onsen, Osaki, Miyagi 989-6711, Japan Tohoku University Osaki Japan.
² Kyushu Open University, 744 Motooka, Fukuoka, 819-0395, Japan Kyushu Open University Fukuoka Japan.
³ The Kagoshima University Museum, Kagoshima University, 1-21-30 Korimoto, Kagoshima, 890-0065, Japan Kagoshima University Kagoshima Japan.
⁴ Department of Environmental Science, University of Human Environments, Okazaki, Aichi, 444-3505, Japan University of Human Environments Okazaki Japan.
⁵ Tsunehisa 5-4-7, Miyazaki 880-0913, Japan Tsunehisa 5-4-7 Miyazaki Japan.

Abstract

According to the contemporary classification of Hydrangea native to Japan, H.serrata is a polymorphic species including six varieties. We discovered a plant identified as H.serrata, but morphologically distinct from previously known varieties, in Yakushima island where approximately 50 endemic species are known. To determine the relationship of this plant with previously known varieties, we examined morphology and constructed a highly resolved phylogeny of H.serrata and its relatives using three chloroplast genomic regions, rbcL, trnL intron, psbA-trnH, and two nuclear genomic regions, ITS1 and ITS2, and Multiplex ISSR genotyping by sequencing (MIG-seq). Based on these morphological and phylogenetic observations, we describe Hydrangeaacuminatasubsp.yakushimensissubsp. nov. as a newly discovered lineage in Yakushima, Japan and propose Hydrangeaminamitanii stat. nov. and Hydrangeaacuminatasubsp.australisstat. nov. which were previously treated as varieties of H.serrata.

Keywords: DNA barcoding; F ST; ITS; MIG-seq; cpDNA; island; threatened plants.

Shun K. Hirota, Tetsukazu Yahara, Kengo Fuse, Hiroyuki Sato, Shuichiro Tagane, Shinji Fujii, Tadashi Minamitani, Yoshihisa Suyama.

Grants and funding

Kyushu Open University