Objective: The aim: The aim of the study was a comparative analysis of indicators characterizing the state of connective tissue in patients with hydronephrosis due to upper urinary tract obstruction, with the presence and absence of recurrence after surgery.
Patients and methods: Materials and methods: Levels of free and bound hydroxyproline, as well as the key mediator of fibrogenesis transforming growth factor-β1 in serum of patients with congenital and acquired obstructions were determined. Ratio peptide-bound and free hydroxyproline were calculated. Groups were divided according to the presence or absence of recurrence of the stricture for a period of 4.5 years after surgery.
Results: Results: Imbalance of the destructive and synthetic processes in extracellular matrix of connective tissue that is characterized by a higher content of fractions of hydroxyproline and transforming growth factor-β1 in the serum were identified. It is shown that the most pronounced changes are observed in patients with relapsing. In patients with a congenital obstruction and a recurrent course of the disease, the highest activation of the collagen metabolism was observed, which was evidenced by the high levels peptide-bound and protein-bound hydroxyproline, relative to these indicators in patients with acquired obstruction (as with the presence and absence of relapses).
Conclusion: Conclusions: The increase in the ratio of peptide-bound/free hydroxyproline and the level of transforming growth factor-β1 in the blood of patients with stage II-III hydronephrosis on the 21st day after surgery may be a prognostic marker for the development of disease recurrence.
Keywords: connective tissue; hydronephrosis; hydroxyproline; transforming growth factor-β1.