Purification of inclusion bodies (IBs) is gaining importance due to the raising of novel applications for these submicron particulate protein clusters, with potential uses in the biomedical and biotechnological fields among others. Here, we present five optimized methods to purify IBs adapting classical procedures to the material nature, as well as the requirements of the producer cell (Gram-negative bacteria, Gram-positive bacteria, or yeast) and the IB final application.
Keywords: Bacterial cell free; Cell disruption; Escherichia coli; Inclusion Body; Lactococcus lactis; Nanoparticles; Pichia pastoris; Purification.
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