Oligomerization of the FliF Domains Suggests a Coordinated Assembly of the Bacterial Flagellum MS Ring

Giuseppina Mariano; Raquel Faba-Rodriguez; Soi Bui; Weilong Zhao; James Ross; Svetomir B Tzokov; Julien R C Bergeron

doi:10.3389/fmicb.2021.781960

Oligomerization of the FliF Domains Suggests a Coordinated Assembly of the Bacterial Flagellum MS Ring

Front Microbiol. 2022 Jan 11:12:781960. doi: 10.3389/fmicb.2021.781960. eCollection 2021.

Authors

Giuseppina Mariano^{1

2}, Raquel Faba-Rodriguez², Soi Bui¹, Weilong Zhao¹, James Ross³, Svetomir B Tzokov², Julien R C Bergeron^{1

2}

Affiliations

¹ Randall Division of Cell and Molecular Biophysics, King's College London, London, United Kingdom.
² Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, United Kingdom.
³ Department of Biochemistry, University of British Columbia, Vancouver, BC, Canada.

Abstract

The bacterial flagellum is a complex, self-assembling macromolecular machine that powers bacterial motility. It plays diverse roles in bacterial virulence, including aiding in colonization and dissemination during infection. The flagellum consists of a filamentous structure protruding from the cell, and of the basal body, a large assembly that spans the cell envelope. The basal body is comprised of over 20 different proteins forming several concentric ring structures, termed the M- S- L- P- and C-rings, respectively. In particular, the MS rings are formed by a single protein FliF, which consists of two trans-membrane helices anchoring it to the inner membrane and surrounding a large periplasmic domain. Assembly of the MS ring, through oligomerization of FliF, is one of the first steps of basal body assembly. Previous computational analysis had shown that the periplasmic region of FliF consists of three structurally similar domains, termed Ring-Building Motif (RBM)1, RBM2, and RBM3. The structure of the MS-ring has been reported recently, and unexpectedly shown that these three domains adopt different symmetries, with RBM3 having a 34-mer stoichiometry, while RBM2 adopts two distinct positions in the complex, including a 23-mer ring. This observation raises some important question on the assembly of the MS ring, and the formation of this symmetry mismatch within a single protein. In this study, we analyze the oligomerization of the individual RBM domains in isolation, in the Salmonella enterica serovar Typhimurium FliF ortholog. We demonstrate that the periplasmic domain of FliF assembles into the MS ring, in the absence of the trans-membrane helices. We also report that the RBM2 and RBM3 domains oligomerize into ring structures, but not RBM1. Intriguingly, we observe that a construct encompassing RBM1 and RBM2 is monomeric, suggesting that RBM1 interacts with RBM2, and inhibits its oligomerization. However, this inhibition is lifted by the addition of RBM3. Collectively, this data suggest a mechanism for the controlled assembly of the MS ring.

Keywords: Salmonella; bacteria; cryo-EM; flagellum; structure.