The role of ambra1 in Pb-induced developmental neurotoxicity in zebrafish

Biochem Biophys Res Commun. 2022 Feb 26:594:139-145. doi: 10.1016/j.bbrc.2021.12.084. Epub 2021 Dec 25.

Abstract

Lead is a highly toxic metal that displays developmental neurotoxicity. Ambra1 plays a crucial role in embryonic neural development. At present, the role of Ambra1 in lead-induced developmental neurotoxicity remains unknown. In this study, we investigated the mechanism of Ambra1 concerning its role in lead-induced neurotoxicity. Zebrafish (Danio rerio) embryos were exposed to 0.1, 1, or 10 μM Pb until 5 days post-fertilization, and their locomotor activity was significantly impaired by the 10 μM treatment. Meanwhile, Pb reduced the expression of ambra1a and ambra1b in the brain at 48 and 72 h post-fertilization. Overexpression of ambra1a or ambra1b reversed Pb-induced alterations in locomotor activity, and decreased the apoptotic cell numbers in the brains of Pb-treated zebrafish. Our data reveal a novel protective role of Ambra1 against Pb-induced neural damage in the developing zebrafish.

Keywords: Ambra1; Lead; Neurotoxicity; Zebrafish.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing / physiology*
  • Animals
  • Apoptosis
  • Brain / drug effects*
  • Brain / embryology*
  • Brain Injuries / metabolism*
  • Dose-Response Relationship, Drug
  • Embryo, Nonmammalian / metabolism
  • Embryonic Development
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental*
  • Gene Silencing
  • In Situ Hybridization
  • Larva
  • Lead*
  • Movement / drug effects*
  • Nervous System
  • Neurogenesis
  • Neurotoxicity Syndromes / metabolism
  • Neurotoxins
  • Zebrafish
  • Zebrafish Proteins / physiology*

Substances

  • Adaptor Proteins, Signal Transducing
  • Ambra1a protein, zebrafish
  • Ambra1b protein, zebrafish
  • Neurotoxins
  • Zebrafish Proteins
  • Lead