Determination of IC50 values of anticancer drugs on cells by D2O - single cell Raman spectroscopy

Wanxue Lv; Boqiang Fu; Manli Li; Yu Kang; Shouli Bai; Chao Lu

doi:10.1039/d1cc06857a

Determination of IC₅₀ values of anticancer drugs on cells by D₂O - single cell Raman spectroscopy

Chem Commun (Camb). 2022 Feb 15;58(14):2355-2358. doi: 10.1039/d1cc06857a.

Authors

Wanxue Lv^{1

2}, Boqiang Fu², Manli Li², Yu Kang¹, Shouli Bai¹, Chao Lu¹

Affiliations

¹ State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China. luchao@mail.buct.edu.cn.
² Center for Advanced Measurement Science (Institute of Life Science Metrology), National Institute of Metrology China, Beijing 100029, China. fubq@nim.ac.cn.

PMID: 35080537
DOI: 10.1039/d1cc06857a

Abstract

A simple, sensitive and repeatable D₂O-single cell Raman spectroscopy method is developed to quantify the inhibitory activity of anticancer drugs on cancer cell metabolism. The IC₅₀ values obtained from A549 cells incubated with cisplatin and taxol are comparable with results of CCK-8 and ATP luminescent cell viability assays.

MeSH terms

A549 Cells
Antineoplastic Agents / chemistry
Antineoplastic Agents / pharmacology*
Cell Survival / drug effects
Cisplatin / chemistry
Cisplatin / pharmacology*
Deuterium Oxide / antagonists & inhibitors
Deuterium Oxide / pharmacology
Dose-Response Relationship, Drug
Drug Screening Assays, Antitumor
Humans
Paclitaxel / chemistry
Paclitaxel / pharmacology*
Single-Cell Analysis*
Spectrum Analysis, Raman
Structure-Activity Relationship

Substances

Antineoplastic Agents
Deuterium Oxide
Paclitaxel
Cisplatin