Extracellular vesicle glucose transporter-1 and glycan features in monocyte-endothelial inflammatory interactions

Man Yang; Sierra A Walker; Jesús S Aguilar Díaz de León; Irina Davidovich; Kelly Broad; Yeshayahu Talmon; Chad R Borges; Joy Wolfram

doi:10.1016/j.nano.2022.102515

Extracellular vesicle glucose transporter-1 and glycan features in monocyte-endothelial inflammatory interactions

Nanomedicine. 2022 Jun:42:102515. doi: 10.1016/j.nano.2022.102515. Epub 2022 Jan 22.

Authors

Man Yang¹, Sierra A Walker², Jesús S Aguilar Díaz de León³, Irina Davidovich⁴, Kelly Broad², Yeshayahu Talmon⁵, Chad R Borges⁶, Joy Wolfram⁷

Affiliations

¹ Department of Biochemistry and Molecular Biology, Department of Physiology and Biomedical Engineering, Department of Transplantation, Mayo Clinic, Jacksonville, FL, USA; School of Public Health and Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, China.
² Department of Biochemistry and Molecular Biology, Department of Physiology and Biomedical Engineering, Department of Transplantation, Mayo Clinic, Jacksonville, FL, USA.
³ School of Molecular Sciences and Virginia G. Piper Center for Personalized Diagnostics, The Biodesign Institute at Arizona State University, Tempe, AZ, USA.
⁴ Department of Chemical Engineering and the Russell Berrie Nanotechnology Institute (RBNI), Technion-Israel Institute of Technology, Haifa, Israel.
⁵ Department of Chemical Engineering and the Russell Berrie Nanotechnology Institute (RBNI), Technion-Israel Institute of Technology, Haifa, Israel. Electronic address: ishi@technion.ac.il.
⁶ School of Molecular Sciences and Virginia G. Piper Center for Personalized Diagnostics, The Biodesign Institute at Arizona State University, Tempe, AZ, USA. Electronic address: chad.borges@asu.edu.
⁷ Department of Biochemistry and Molecular Biology, Department of Physiology and Biomedical Engineering, Department of Transplantation, Mayo Clinic, Jacksonville, FL, USA; Department of Nanomedicine, Houston Methodist Research Institute, Houston, TX, USA; Australian Institute for Bioengineering and Nanotechnology, University of Queensland, Brisbane, QLD, Australia (present affiliation); School of Chemical Engineering, University of Queensland, Brisbane, QLD, Australia (present affiliation). Electronic address: j.wolfram@uq.edu.au.

PMID: 35074500
DOI: 10.1016/j.nano.2022.102515

Abstract

Monocyte-induced endothelial cell inflammation is associated with multiple pathological conditions, and extracellular vesicles (EVs) are essential nanosized components of intercellular communication. EVs derived from endotoxin-stimulated monocytes were previously shown to carry pro-inflammatory proteins and RNAs. The role of glucose transporter-1 (GLUT-1) and glycan features in monocyte-derived EV-induced endothelial cell inflammation remains largely unexplored. This study demonstrates that EVs derived from endotoxin-stimulated monocytes activate inflammatory pathways in endothelial cells, which are partially attributed to GLUT-1. Alterations in glycan features and increased levels of GLUT-1 were observed in EVs derived from endotoxin-stimulated monocytes. Notably, inhibition of EV-associated GLUT-1, through the use of fasentin, suppressed EV-induced inflammatory cytokines in recipient endothelial cells.

Keywords: Exosome; Extracellular vesicle; Glucose transporter-1; Glycan node analysis; Inflammation.

MeSH terms

Endothelial Cells / metabolism
Endothelial Cells / pathology
Endotoxins / pharmacology
Extracellular Vesicles* / metabolism
Glucose Transporter Type 1* / metabolism
Humans
Inflammation* / metabolism
Inflammation* / pathology
Monocytes* / metabolism
Monocytes* / pathology
Polysaccharides* / metabolism

Substances

Endotoxins
Glucose Transporter Type 1
Polysaccharides
SLC2A1 protein, human