Modification of chitosan nanofibers with CuS and fucoidan for antibacterial and bone tissue engineering applications

Hsien-Tsung Lu; Guan-Yu Huang; Wong-Jin Chang; Tzu-Wei Lu; Tzu-Wen Huang; Ming-Hua Ho; Fwu-Long Mi

doi:10.1016/j.carbpol.2021.119035

Modification of chitosan nanofibers with CuS and fucoidan for antibacterial and bone tissue engineering applications

Carbohydr Polym. 2022 Apr 1:281:119035. doi: 10.1016/j.carbpol.2021.119035. Epub 2022 Jan 7.

Authors

Hsien-Tsung Lu¹, Guan-Yu Huang², Wong-Jin Chang³, Tzu-Wei Lu⁴, Tzu-Wen Huang⁵, Ming-Hua Ho⁶, Fwu-Long Mi⁷

Affiliations

¹ Department of Orthopedics, Taipei Medical University Hospital, Taipei City 11031, Taiwan; Department of Orthopedics, School of Medicine, College of Medicine, Taipei Medical University, Taipei City 11031, Taiwan.
² Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei City 106335, Taiwan.
³ Department of Biochemistry and Molecular Cell Biology, School of Medicine, College of Medicine, Taipei Medical University, Taipei City 11031, Taiwan.
⁴ Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei City 11031, Taiwan.
⁵ Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei City 11031, Taiwan.
⁶ Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei City 106335, Taiwan. Electronic address: mhho@mail.ntust.edu.tw.
⁷ Department of Biochemistry and Molecular Cell Biology, School of Medicine, College of Medicine, Taipei Medical University, Taipei City 11031, Taiwan; Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei City 11031, Taiwan; Graduate Institute of Nanomedicine and Medical Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei City 11031, Taiwan. Electronic address: flmi530326@tmu.edu.tw.

PMID: 35074103
DOI: 10.1016/j.carbpol.2021.119035

Abstract

Chitosan (CS) electrospun nanofiber (ENF) membranes were modified with fucoidan (Fu) and CuS NPs through polyelectrolyte complexation and genipin (GP)-involved cross-linking reaction. The formation of Fu/CS complex and cross-linking of CS with GP increased the acid resistance and reduced the swelling rate of CS ENF, while the covalent conjugation of CuS NPs provided CS ENF with durable Fenton-like catalytic activity. The CuS@ENF composite (ENFC) effectively adsorbed H₂O₂ and near-infrared (NIR) light, enabling it to kill bacteria by photothermal and photocatalytic bactericidal effects. Fu and copper ions were able to release from the ENFC in a pH-dependent manner, and promoted the alkaline phosphatase activity of osteoblast cells and capillary tube formation of endothelial cells. This study provides a new approach to modify CS ENF with antibacterial and osteoblast differentiation activities, which may be available for bone infection prevention and tissue regeneration.

Keywords: Antibacterial; Bone tissue regeneration; Chitosan; CuS nanoparticles; Nanofibers; Photocatalytic; Photothermal; Wound dressing.

MeSH terms

Anti-Bacterial Agents / pharmacology
Chitosan* / pharmacology
Copper
Endothelial Cells
Hydrogen Peroxide
Nanofibers*
Polysaccharides
Tissue Engineering

Substances

Anti-Bacterial Agents
Polysaccharides
Copper
Chitosan
fucoidan
Hydrogen Peroxide
cupric sulfide