The development and application of new natural selenium polysaccharides with relatively clear structure and excellent activity have become hot and difficult issues. This study used GC-MS and 2D NMR to characterize the detailed chain structure information of selenium polysaccharide (Se-POP-3) from Selenium-enriched Pleurotus ostreatus, and then explored its anti-gastric cancer and anti-colon cancer effects in vitro. Results showed that the main chain of Se-POP-3 was →[3)-β-D-Glcp-(1]2 → 6)-β-D-Glcp-(1 → 3,6)-β-D-Glcp-(1 → 3)-β-D-Glcp-(1→, and the branch was α-D-Glcp-(1 → [4)-α-D-Glcp-(1]4→, which was connected to the main chain through the O-3 bond of →3,6)-β-D-Glcp-(1 → glycosidic bond. In addition, Se-POP-3 could reduce viability, induce apoptosis, inhibit migration and invasion, destroy the Bax/Bcl-2 ratio, and inhibit the epithelial-to-mesenchymal transition of MGC-803 and HCT-116 cells in vitro. Moreover, this study also showed that within the concentration range set in this study, Se-POP-3 had no significant effect on the growth of normal cells (NCM460 cells). This study can provide a theoretical basis for the potential application of Se-POP-3 as an anti-gastrointestinal cancer drug or functional food.
Keywords: Antitumor activity; Pleurotus ostreatus; Selenium-enriched polysaccharide.
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