Wavelength-regulated switchable photoelectrochemical system for concurrent detection of dual antibiotics

Ding Jiang; Yude Zhang; Xiaojiao Du; Youhua Tan; Wen Chen; Mo Yang

doi:10.1016/j.bios.2022.113999

Wavelength-regulated switchable photoelectrochemical system for concurrent detection of dual antibiotics

Biosens Bioelectron. 2022 Apr 15:202:113999. doi: 10.1016/j.bios.2022.113999. Epub 2022 Jan 19.

Authors

Ding Jiang¹, Yude Zhang², Xiaojiao Du³, Youhua Tan⁴, Wen Chen⁵, Mo Yang⁶

Affiliations

¹ Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology, School of Petrochemical Engineering, Changzhou University, Changzhou, Jiangsu, 213164, PR China; Department of Biomedical Engineering, The Hong Kong Polytechnic University, Kowloon, 999077, Hong Kong, SAR, PR China.
² Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology, School of Petrochemical Engineering, Changzhou University, Changzhou, Jiangsu, 213164, PR China.
³ Oakland International Associated Laboratory, School of Photoelectric Engineering, Changzhou Institute of Technology, Changzhou, Jiangsu, 213032, PR China.
⁴ Department of Biomedical Engineering, The Hong Kong Polytechnic University, Kowloon, 999077, Hong Kong, SAR, PR China.
⁵ Department of Electronic and Information Engineering, The Hong Kong Polytechnic University, Kowloon, 999077, Hong Kong, SAR, PR China.
⁶ Department of Biomedical Engineering, The Hong Kong Polytechnic University, Kowloon, 999077, Hong Kong, SAR, PR China. Electronic address: Mo.Yang@polyu.edu.hk.

PMID: 35065476
DOI: 10.1016/j.bios.2022.113999

Abstract

Concurrent detection of antibiotics with high sensitivity and reliability is always of high importance for food safety and environmental monitoring. Herein, a wavelength-dependent photoelectrochemical (PEC) aptasensor based on TiO₂-Ag/nitrogen doped graphene composites (TiO₂-Ag/NDG) was developed for concurrent detection of two antibiotics with signal amplification function. The as-fabricated ternary nanocomposites could concurrently detect two antibiotics using two different aptamer molecules as recognition elements, amplify the photocurrent outputs and perform a photocurrent-switchable operation, where photocurrents could be switched between anodic direction and cathodic direction via simply regulating the irradiation wavelength. Such proposed wavelength-dependent PEC sensing strategy was capable of concurrently detecting chloramphenicol (CAP) with a wide linear detection range from 50 pM-10 nM under irradiation of 380 nm and tetracycline (TET) with a linear detection range from 100 pM to 100 nM under irradiation of 600 nm, respectively. The limits of detection (LOD) for CAP and TET were 16.7 pM and 30 pM, respectively. Moreover, this PEC sensor also displayed good specificity and outstanding reliability in real sample applications. This wavelength-dependent PEC strategy could be conveniently adapted to other applications in food safety biosensing and environmental monitoring.

Keywords: Antibiotic; Nitrogen-doped graphene composites; Photoelectrochemical sensing; TiO(2).

MeSH terms

Anti-Bacterial Agents
Aptamers, Nucleotide* / chemistry
Biosensing Techniques*
Electrochemical Techniques
Graphite* / chemistry
Limit of Detection
Reproducibility of Results

Substances

Anti-Bacterial Agents
Aptamers, Nucleotide
Graphite