Cryo-EM in molecular and cellular biology

Mol Cell. 2022 Jan 20;82(2):274-284. doi: 10.1016/j.molcel.2021.12.016.

Abstract

This review summarizes the current state of methods and results achievable by cryo-electron microscopy (cryo-EM) imaging for molecular, cell, and structural biologists who wish to understand what is required and how it might help to address their research questions. It covers some of the main issues in sample preparation, microscopes and data collection, image processing, three-dimensional (3D) reconstruction, and validation and interpretation of the resulting EM density maps and atomic models.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Cell Biology*
  • Cryoelectron Microscopy*
  • Data Collection
  • Electron Microscope Tomography
  • Histocytological Preparation Techniques
  • Humans
  • Image Processing, Computer-Assisted
  • Models, Molecular
  • Molecular Biology*
  • Single Molecule Imaging
  • Specimen Handling