A bifunctional fluorescent probe based on "AND logic" for the simultaneous recognition of H2S/HNO and its bioimaging applications

Huawei Niu; Xintong Mi; Xinting Hua; Yuanyuan Zhang; Yaping Zhai; Fangyuan Qin; Yong Ye; Yufen Zhao

doi:10.1016/j.aca.2021.339341

A bifunctional fluorescent probe based on "AND logic" for the simultaneous recognition of H₂S/HNO and its bioimaging applications

Anal Chim Acta. 2022 Feb 1:1192:339341. doi: 10.1016/j.aca.2021.339341. Epub 2021 Nov 30.

Authors

Huawei Niu¹, Xintong Mi², Xinting Hua², Yuanyuan Zhang², Yaping Zhai³, Fangyuan Qin⁴, Yong Ye⁵, Yufen Zhao⁶

Affiliations

¹ College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, 471000, China. Electronic address: niuhw0816@126.com.
² College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, 471000, China.
³ Institute of Ophthalmology, Henan Provincial People's Hospital, Zhengzhou, 450003, China.
⁴ Institute of Ophthalmology, Henan Provincial People's Hospital, Zhengzhou, 450003, China. Electronic address: 12371369@qq.com.
⁵ Green Catalysis Center, and College of Chemistry, Zhengzhou University, Zhengzhou, 450001, China. Electronic address: yeyong03@tsinghua.org.cn.
⁶ Institute of Drug Discovery Technology, Ningbo University, Ningbo, 450052, China.

PMID: 35057948
DOI: 10.1016/j.aca.2021.339341

Abstract

The reaction of NO and H₂S to form HNO is a classical pathway in physiological conditions. The reported single recognition-type fluorescent probes are difficult to track precisely the relationships of H₂S and HNO. It is necessary to develop a bifunctional fluorescence probe (NJA) for monitoring simultaneously the production of endogenous HNO and H₂S. Using 7-Nitrobenzofurazan (NBD) and 2-(diphenylphosphine) benzoate as recognition sites, the obatined NJA can detect specifically HS^- and HNO. The detection limit of HS^- and HNO are 0.46 μM and 1.42 μM, respectively. Based on the dual recognition sites and input signals of the probe, a molecular "AND" logic gate was established to detect successfully H₂S and HNO in MCF-7 cells. NJA based on "AND logic" provided a simple and robust tool for monitoring the production of endogenous HNO correlative with H₂S and NO in living cells.

Keywords: Bifunctional; Fluorescence probe; Hydrogen sulfide; Logic gate; Nitroxyl.

MeSH terms

Fluorescent Dyes*
Humans
MCF-7 Cells
Nitrogen Oxides*

Substances

Fluorescent Dyes
Nitrogen Oxides