Abstract
Fungal laccase obtained from a Cerrena unicolor strain was used as an effective biocatalyst for the transformation of 8-anilino-1-naphthalenesulfonic acid into a green-coloured antibacterial compound, which can be considered as both an antimicrobial agent and a textile dye, simultaneously. The process of biosynthesis was performed in buffered solutions containing methanol as a co-solvent, allowing better solubilisation of substrate. The transformation process was optimised in terms of the buffer pH value, laccase activity, and concentrations of the substrate and co-solvent. The crude product obtained exhibited low cytotoxicity, antibacterial properties against Staphylococcus aureus and Staphylococcus epidermidis, and antioxidant properties. Moreover, the synthesised green-coloured compound proved non-allergenic and demonstrated a high efficiency of dyeing wool fibres.
Keywords:
bioactive compounds; biocatalysis; laccase.
MeSH terms
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Adult
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Aged
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Aliivibrio fischeri / drug effects
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Anilino Naphthalenesulfonates / chemistry
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Anilino Naphthalenesulfonates / metabolism*
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Anti-Bacterial Agents / biosynthesis
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Anti-Bacterial Agents / chemistry*
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Anti-Bacterial Agents / pharmacology*
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Anti-Bacterial Agents / toxicity
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Antioxidants / chemistry
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Antioxidants / metabolism
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Antioxidants / pharmacology
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Antioxidants / toxicity
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Biocatalysis
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Cell Line
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Colon / drug effects
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Coloring Agents / chemistry*
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Coloring Agents / metabolism
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Coloring Agents / pharmacology*
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Coloring Agents / toxicity
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Epithelial Cells / drug effects
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Female
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Fibroblasts / drug effects
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Fungi / enzymology
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Healthy Volunteers
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Humans
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Hypersensitivity
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Laccase / chemistry
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Laccase / metabolism*
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Male
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Middle Aged
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Oxidation-Reduction
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Skin / drug effects
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Staphylococcus aureus / drug effects
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Staphylococcus epidermidis / drug effects
Substances
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8-anilino-1-naphthalenesulfonic acid
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Anilino Naphthalenesulfonates
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Anti-Bacterial Agents
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Antioxidants
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Coloring Agents
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Laccase