Solid lipid nanoparticles loaded with curcumin: development and in vitro toxicity against CT26 cells

Rayane Ganassin; Victor Carlos Mello da Silva; Victor Hugo Sousa Araujo; Giulia Rosa Tavares; Patrícia Bento da Silva; Paolin Rocio Cáceres-Vélez; Joaquin E Martínez Porcel; Mosar Corrêa Rodrigues; Patrizia Andreozzi; Richard Perosa Fernandes; Bruno Fonseca-Santos; Sergio Moya; Ricardo Bentes Azevedo; Marlus Chorilli; Luis Alexandre Muehlmann

doi:10.2217/nnm-2021-0229

Solid lipid nanoparticles loaded with curcumin: development and in vitro toxicity against CT26 cells

Nanomedicine (Lond). 2022 Feb;17(3):167-179. doi: 10.2217/nnm-2021-0229. Epub 2022 Jan 20.

Authors

Rayane Ganassin^{1

2}, Victor Carlos Mello da Silva^{1

2}, Victor Hugo Sousa Araujo^{1

2}, Giulia Rosa Tavares^{1

2}, Patrícia Bento da Silva², Paolin Rocio Cáceres-Vélez^{2

3

4}, Joaquin E Martínez Porcel³, Mosar Corrêa Rodrigues^{1

2}, Patrizia Andreozzi³, Richard Perosa Fernandes⁵, Bruno Fonseca-Santos⁶, Sergio Moya³, Ricardo Bentes Azevedo², Marlus Chorilli⁷, Luis Alexandre Muehlmann^{1

2}

Affiliations

¹ Laboratory of Nanoscience & Immunology, Faculty of Ceilandia, University of Brasilia, Brasilia/DF, 72220-900, Brazil.
² Department of Genetics & Morphology, Institute of Biological Sciences, University of Brasilia, Brasilia, 70910-900, Brazil.
³ Soft Matter Laboratory, Center for Cooperative Research in Biomaterials (CIC biomaGUNE), San Sebastian, Spain.
⁴ School of Biosciences, The University of Melbourne, Parkville, VIC 3010, Australia.
⁵ Laboratory of Thermal Analysis Ivo Giolitto, Chemical Institute, São Paulo State University São Paulo, Araraquara -SP, Brazil.
⁶ University of Campinas (UNICAMP), 13083-871 Campinas, São Paulo, Brazil.
⁷ School of Pharmaceutical Sciences, São Paulo State University (UNESP), 14800-903, Araraquara, Brazil.

PMID: 35048742
DOI: 10.2217/nnm-2021-0229

Abstract

Aim: To develop a new curcumin carrier consisting of murumuru butter nanoparticles (SLN-Cs). Methods: A phase-inversion temperature method was used to produce SLN-Cs. The interaction of SLN-Cs with murine colon adenocarcinoma (CT26) cells in vitro was analyzed by confocal microscopy. Results: Stable SLN-Cs with a high curcumin-loading capacity were obtained. The SLN-Cs were more toxic to CT26 than free curcumin. Fluorescence microscopy images showed the SLN-Cs to be taken up by CT26 cells in vitro. Conclusion: These results indicate that SLN-Cs are suitable carriers of curcumin in aqueous media.

Keywords: cancer; colloid; colon adenocarcinoma; drug delivery; murumuru butter.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Curcumin*
Drug Carriers
Lipids
Liposomes
Mice
Nanoparticles* / toxicity
Particle Size

Substances

Drug Carriers
Lipid Nanoparticles
Lipids
Liposomes
Curcumin