Rates of pyruvate carboxylase, glutamate and GABA neurotransmitter cycling, and glucose oxidation in multiple brain regions of the awake rat using a combination of [2-13C]/[1-13C]glucose infusion and 1H-[13C]NMR ex vivo

J Cereb Blood Flow Metab. 2022 Aug;42(8):1507-1523. doi: 10.1177/0271678X221074211. Epub 2022 Jan 20.

Abstract

Anaplerosis occurs predominately in astroglia through the action of pyruvate carboxylase (PC). The rate of PC (Vpc) has been reported for cerebral cortex (or whole brain) of awake humans and anesthetized rodents, but regional brain rates remain largely unknown and, hence, were subjected to investigation in the current study. Awake male rats were infused with either [2-13C]glucose or [1-13C]glucose (n = 27/30) for 8, 15, 30, 60 or 120 min, followed by rapid euthanasia with focused-beam microwave irradiation to the brain. Blood plasma and extracts of cerebellum, hippocampus, striatum, and cerebral cortex were analyzed by 1H-[13C]-NMR to establish 13C-enrichment time courses for glutamate-C4,C3,C2, glutamine-C4,C3, GABA-C2,C3,C4 and aspartate-C2,C3. Metabolic rates were determined by fitting a three-compartment metabolic model (glutamatergic and GABAergic neurons and astroglia) to the eighteen time courses. Vpc varied by 44% across brain regions, being lowest in the cerebellum (0.087 ± 0.004 µmol/g/min) and highest in striatum (0.125 ± 0.009) with intermediate values in cerebral cortex (0.106 ± 0.005) and hippocampus (0.114 ± 0.005). Vpc constituted 13-19% of the oxidative glucose consumption rate. Combination of cerebral cortical data with literature values revealed a positive correlation between Vpc and the rates of glutamate/glutamine-cycling and oxidative glucose consumption, respectively, consistent with earlier observations.

Keywords: Anaplerosis; cerebellum; cerebral cortex; hippocampus; striatum.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Brain / metabolism
  • Carbon Isotopes / metabolism
  • Glucose / metabolism
  • Glutamic Acid* / metabolism
  • Glutamine / metabolism
  • Male
  • Neurons / metabolism
  • Neurotransmitter Agents / metabolism
  • Pyruvate Carboxylase* / metabolism
  • Rats
  • Wakefulness
  • gamma-Aminobutyric Acid / metabolism

Substances

  • Carbon Isotopes
  • Neurotransmitter Agents
  • Glutamine
  • Glutamic Acid
  • gamma-Aminobutyric Acid
  • Pyruvate Carboxylase
  • Glucose