Wickerhamiella nakhonpathomensis f.a. sp. nov., a novel ascomycetous yeast species isolated from a mushroom and a flower in Thailand

Pannida Khunnamwong; Kanokwan Kingphadung; Thanasak Lomthong; Apinun Kanpiengjai; Chartchai Khanongnuch; Savitree Limtong

doi:10.1099/ijsem.0.005191

Wickerhamiella nakhonpathomensis f.a. sp. nov., a novel ascomycetous yeast species isolated from a mushroom and a flower in Thailand

Int J Syst Evol Microbiol. 2022 Jan;72(1). doi: 10.1099/ijsem.0.005191.

Authors

Pannida Khunnamwong^{1

2}, Kanokwan Kingphadung³, Thanasak Lomthong⁴, Apinun Kanpiengjai⁵, Chartchai Khanongnuch⁶, Savitree Limtong^{1

2

7}

Affiliations

¹ Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
² Biodiversity Center Kasetsart University (BDCKU), Bangkok 10900, Thailand.
³ Department of Food Technology, Faculty of Engineering and Industrial Technology, Silpakorn University, Nakhon Pathom 73000, Thailand.
⁴ Division of Biology, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, Pathum Thani 12110, Thailand.
⁵ Division of Biochemistry and Biochemical Technology, Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
⁶ Division of Biotechnology, Faculty of Agro-Industry, Chiang Mai University, Mae-Hia, Chiang Mai 50100, Thailand.
⁷ Academy of Science, Royal Society of Thailand, Bangkok 10300, Thailand.

PMID: 35044902
DOI: 10.1099/ijsem.0.005191

Abstract

Strains SU22^T (TBRC 14875^T) and FLA11.5, representing a novel anamorphic yeast species, were respectively isolated from a fruiting body of a Coprinus species and an inflorescence of a Coffea species collected in Thailand. Analysis of the sequences of the D1/D2 domains of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) regions showed that the two strains differed by two nucleotide substitutions in the D1/D2 domains of the LSU rRNA gene and were identical in the ITS regions. Wickerhamiella drosophilae CBS 8459^T was the most closely related species, but with 24-26 nucleotide substitutions in the D1/D2 domains of the LSU rRNA gene and 24 nucleotide substitutions in the ITS regions. A phylogenetic analysis, based on the sequences of the D1/D2 domains, indicated that the two strains represented a species in the genus Wickerhamiella which was distinct from other recognized species of the genus. Therefore, the two strains were assigned as a novel species, for which we propose the name Wickerhamiella nakhonpathomensis f.a. sp. nov. The holotype is TBRC 14875^T (isotype PYCC 8914^T). The MycoBank number of the novel species is MB 840833.

Keywords: Thailand; Wickerhamiella nakhonpathomensis f.a. sp. nov.; inflorescence; mushroom; yeast.

MeSH terms

Agaricales*
DNA, Fungal / genetics
DNA, Ribosomal Spacer / genetics
Fatty Acids / chemistry
Flowers / microbiology*
Mycological Typing Techniques
Phylogeny*
Saccharomycetales* / classification
Saccharomycetales* / isolation & purification
Sequence Analysis, DNA
Thailand

Substances

DNA, Fungal
DNA, Ribosomal Spacer
Fatty Acids