Acetolactate synthase (AHAS) catalyses the first common step in the biosynthesis pathways of three branched-chain amino acids (BCAAs) of valine, isoleucine and leucine. Here, we characterized one regulatory subunit (VdILV6) and three catalytic subunits (VdILV2A, VdILV2B and VdILV2C) of AHAS from the important cotton Verticillium wilt fungus Verticillium dahliae. Phenotypic analysis showed that VdILV6 knockout mutants were auxotrophic for valine and isoleucine and were defective in conidial morphogenesis, hypha penetration and virulence to cotton, and lost ability of microscletotial formation. The growth of single catalytic subunit gene knockout mutants were significantly inhibited by leucine at higher concentration and single catalytic subunit gene knockout mutants showed significantly reduced virulence to cotton. VdILV2B knockout also led to obviously reduced microscletotial formation and conidial production, VdILV2C knockout led to reduced conidial production. Further studies suggested that both feedback inhibition by leucine and the inhibition by AHAS inhibiting herbicides of tribenuron and bispyribac resulted in significantly down-regulated expression of the four subunit VdILVs genes (VdILV2A, VdILV2B, VdILV2C and VdILV6). Any single catalytic subunit gene knockout led to reduced expression of the other three subunit genes, whereas VdILV6 knckout induced increased expression of the three catalytic subunit genes. VdILV2B, VdILV2C and VdILV6 knockout resulted in increased expression of VdCPC1 regulator gene of the cross-pathway control of amino acid biosynthesis. Taken together, these results indicate multiple roles of four VdILVs genes in the biosynthesis of BCAAs, virulence, fungal growth and development in the filamentous fungi V. dahliae.
Keywords: Acetolactate synthase; Pathogenicity genes; Verticillium dahliae.
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