Native-PAGE analysis of protein aggregation upon viral infection in mouse macrophages

Zhenzhen Yan; Hansen Liu; Chengjiang Gao

doi:10.1016/j.xpro.2021.101080

Native-PAGE analysis of protein aggregation upon viral infection in mouse macrophages

STAR Protoc. 2022 Jan 8;3(1):101080. doi: 10.1016/j.xpro.2021.101080. eCollection 2022 Mar 18.

Authors

Zhenzhen Yan¹, Hansen Liu¹, Chengjiang Gao¹

Affiliation

¹ Key Laboratory of Infection and Immunity of Shandong Province & Department of Immunology, School of Biomedical Sciences, Shandong University, Jinan, Shandong 250012, China.

Abstract

Upon viral infection, several proteins in the innate signaling pathway form aggregates, which in turn promote the activation of innate antiviral immune response. In this protocol, we use herpes simplex virus type 1 (HSV-1) to infect mouse peritoneal macrophages, and show how to detect the aggregation of TBK1 upon viral infection. The protocol is adaptable for other proteins and other viruses. For complete details on the use and execution of this profile, please refer to Yan et al. (2021).

Keywords: Cell Biology; Cell isolation; Immunology; Microbiology; Protein Biochemistry.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Herpesvirus 1, Human*
Host-Pathogen Interactions
Macrophages
Mice
Protein Aggregates
Virus Diseases*

Substances

Protein Aggregates