Translation regulatory long non-coding RNA 1 (TRERNA1) sponges microRNA-23a to suppress granulosa cell apoptosis in premature ovarian failure

Bioengineered. 2022 Feb;13(2):2173-2180. doi: 10.1080/21655979.2021.2023802.

Abstract

Translation regulatory long non-coding RNA 1 (TRERNA1) plays critical roles in cancer biology. We predicted the direct interaction of TRERNA1 with microRNA (miR)-23a, which promotes granulosa apoptosis. Granulosa apoptosis is involved in premature ovarian failure (POF). This study was therefore carried out to explore the involvement of TRERNA1 and miR-23a in POF. The expression of TRERNA1 and miR-23a in POF and control groups were detected by RT-qPCRs. The subcellular locations of TRERNA1 in granulosa cell line COV434 was detected by subcellular fractionation assay. The interaction between TRERNA1 and miR-23a was predicted using IntaRNA2.0. The direct interaction between COV434 and miR-23a was explored with RNA pull-down assay. In granulosa cells, the direct interaction between TRERNA1 and miR-23a was verified by overexpression assay. Cell apoptosis assay was performed to evaluate cell apoptosis. Both TRERNA1 and miR-23a were downregulated in POF. In addition, TRERNA1 was detected in both cytoplasm and nuclear samples of granulosa cells, and directly interacted with miR-23a. TRERNA1 did not affect the expression of miR-23a in granulosa cells, while TRERNA1 suppressed the role of miR-23a in enhancing cell apoptosis. In conclusion, TRERNA1 may sponge miR-23a to suppress granulosa cell apoptosis in POF.

Keywords: TRERNA1; apoptosis; miR-23a; premature ovarian failure.

Publication types

  • Research Support, Non-U.S. Gov't
  • Video-Audio Media

MeSH terms

  • Adult
  • Apoptosis*
  • Cell Line, Tumor
  • Female
  • Granulosa Cells / metabolism*
  • Granulosa Cells / pathology
  • Humans
  • MicroRNAs / genetics
  • MicroRNAs / metabolism*
  • Primary Ovarian Insufficiency / genetics
  • Primary Ovarian Insufficiency / metabolism*
  • Primary Ovarian Insufficiency / pathology
  • RNA, Long Noncoding / genetics
  • RNA, Long Noncoding / metabolism*

Substances

  • MIRN23a microRNA, human
  • MicroRNAs
  • RNA, Long Noncoding
  • tre long noncoding RNA, human

Grants and funding

We thank the financial support from Natural Science Foundation of Gansu Province, No. 20JR10RA696, 21JR1RA067; The First Hospital of Lanzhou University, No.ldyyyn 2018-68; The Innovation Ability Enhancement Project of Higher Education Institutions of Gansu Province, No. 2020B-012.