Diagnostic value of long noncoding RNA LINC01485 in patients with colorectal cancer

Zuojian Hu; Junrong Wu; Shaolin Tan; Lujie Yang; Junhui Huang; Cuiju Mo; Huaping Chen; Xuelian Ruan; Xue Qin

doi:10.1016/j.clinbiochem.2022.01.003

Diagnostic value of long noncoding RNA LINC01485 in patients with colorectal cancer

Clin Biochem. 2022 Apr:102:34-43. doi: 10.1016/j.clinbiochem.2022.01.003. Epub 2022 Jan 13.

Authors

Zuojian Hu¹, Junrong Wu², Shaolin Tan³, Lujie Yang⁴, Junhui Huang¹, Cuiju Mo¹, Huaping Chen¹, Xuelian Ruan¹, Xue Qin⁵

Affiliations

¹ Department of Laboratory Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi, China.
² Department of Laboratory Medicine, The Affiliated Tumor Hospital of Guangxi Medical University, Nanning 530021, Guangxi, China.
³ Department of Orthopaedics, The Second Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi, China.
⁴ Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China.
⁵ Department of Laboratory Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi, China. Electronic address: qinxue@gxmu.edu.cn.

PMID: 35033545
DOI: 10.1016/j.clinbiochem.2022.01.003

Abstract

Background: Long noncoding RNAs (lncRNAs) were considered as transcription noise without biological functions. However, accumulated evidence shows that lncRNAs are expressed heterogeneously in tumor tissues. This study aims to identify the specific expression of lncRNAs in colorectal cancer patients and to perform verification analysis.

Methods: The differentially expressed lncRNAs and mRNAs in colorectal cancer and normal tissues were screened by bioinformatics methods. Subsequently, the qRT-PCR method was used to verify the expression of differential lncRNAs in tumor tissues and blood samples. Concurrently ROC curves were used to analyze the diagnostic efficacy of lncRNAs. Moreover, the correlation between lncRNAs and clinicopathological features was also analyzed. Finally, functional annotation analysis was performed for lncRNAs.

Results: Eleven lncRNAs differentially expressed in colorectal cancer tissues and normal tissues were screened. In the validation tissue sample set, FOXD3-AS1 was down-regulated in colorectal cancer tissues (P < 0.001), while LINC01485 was up-regulated in colorectal cancer tissues compared with the adjacent tissues (P < 0.05). In a further verification of the whole blood sample set, LINC01485 showed high sensitivity and specificity (sensitivity = 98.33%, specificity = 84.00%) in differentiating colorectal cancer patients from healthy controls (P < 0.001). Simultaneously, there was no difference in the expression of LINC01485 in other gastrointestinal tumors (hepatocellular carcinoma, esophageal cancer, gastric cancer, and pancreatic cancer) and healthy controls. LINC01485 is significantly related to the clinical staging, lymph node metastasis, and distant metastasis of colorectal cancer.

Conclusions: The expression, diagnostic efficiency, and functional analysis of the lncRNA file of colorectal cancer reveals the important role of LINC01485 in colorectal cancer and provides an important clinical reference value for the early diagnosis and targeted therapy of colorectal cancer.

Keywords: Clinical characteristics; Colorectal cancer; Diagnostic; LINC01485; Whole blood sample.

MeSH terms

Biomarkers, Tumor / genetics
Carcinoma, Hepatocellular* / genetics
Colorectal Neoplasms* / diagnosis
Colorectal Neoplasms* / genetics
Colorectal Neoplasms* / pathology
Forkhead Transcription Factors / genetics
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Humans
Liver Neoplasms* / pathology
RNA, Long Noncoding* / genetics

Substances

Biomarkers, Tumor
FOXD3 protein, human
Forkhead Transcription Factors
RNA, Long Noncoding