Gene Expression of Osteoblast-like Cells on Carbon-Nanowall as Scaffolds during Incubation with Electrical Stimulation

Tomonori Ichikawa; Hiroki Kondo; Kenji Ishikawa; Takayoshi Tsutsumi; Hiromasa Tanaka; Makoto Sekine; Masaru Hori

doi:10.1021/acsabm.9b00178

Gene Expression of Osteoblast-like Cells on Carbon-Nanowall as Scaffolds during Incubation with Electrical Stimulation

ACS Appl Bio Mater. 2019 Jul 15;2(7):2698-2702. doi: 10.1021/acsabm.9b00178. Epub 2019 Jun 12.

Authors

Tomonori Ichikawa¹, Hiroki Kondo¹, Kenji Ishikawa¹, Takayoshi Tsutsumi¹, Hiromasa Tanaka¹, Makoto Sekine¹, Masaru Hori¹

Affiliation

¹ Nagoya University, Furo-cho, Chikusa, Nagoya 464-8601, Japan.

PMID: 35030804
DOI: 10.1021/acsabm.9b00178

Abstract

Nanostructured cell-culture scaffolds of carbon nanowalls (CNWs) were prepared by changing average wall-to-wall distances either 132 or 220 nm. Osteoblast-like cells (Saos-2) proliferated during 4 day incubation on the wider (220 nm) CNW scaffolds in the presence of electrical stimulation (ES). Differentiation gene expression levels of Runt-related transcription factor 2 (Runx2) and osteocalcin (OC) were suppressed after 10 day incubation, which indicated that the average wall-to-wall distances of the CNW scaffolds affect suppression of Runx2 and OC gene expression. This technique holds promise for controlling the differentiation of osteoblast-like cells.

Keywords: Saos-2; carbon nanowall; cell culture scaffolds; electrical stimulation; osteogenic differentiation genes.