In vitro treatment of human granulosa cells with irisin and leptin: Quantitative RT-PCR array data (female infertility panel)

Radoslav Stojchevski; Tomer Singer; Karina Ziskovich; Leonid Poretsky; Dimiter Avtanski

doi:10.1016/j.dib.2021.107781

In vitro treatment of human granulosa cells with irisin and leptin: Quantitative RT-PCR array data (female infertility panel)

Data Brief. 2022 Jan 1:40:107781. doi: 10.1016/j.dib.2021.107781. eCollection 2022 Feb.

Authors

Radoslav Stojchevski¹, Tomer Singer², Karina Ziskovich¹, Leonid Poretsky^{1

3

4}, Dimiter Avtanski^{1

3

4}

Affiliations

¹ Friedman Diabetes Institute, Northwell Health, New York, NY, USA.
² Shady Grove Fertility Clinic, New York, NY, USA.
³ Feinstein Institutes for Medical Research, Northwell Health, Manhasset, NY, USA.
⁴ Donald and Barbara Zucker School of Medicine at Hofstra/Northwell, Hempstead, NY, USA.

Abstract

Reproduction is closely related to energy metabolism: physical activity and adiposity (either insufficient weight or obesity) can affect female fertility. Irisin is a myo- and adipokine produced by skeletal muscles during exercise or shivering as well as in smaller amounts by subcutaneous visceral adipocytes [1]. Leptin is a neuroendocrine adipokine regulating satiety and energy expenditure. Circulating levels of both, irisin and leptin, correlate with adiposity status and physical activity [2], [3], [4], [5], [6]. This article presents data from quantitative PCR array of the in vitro effects of irisin and leptin on cultured human ovarian granulosa cells. Granulosa cells were purified from follicular fluid samples obtained from women undergoing in vitro fertilization (IVF) procedure and were subjected to treatment with irisin (500 ng/mL) or leptin (100 ng/mL) for 24 h. The array included 84 genes involved in female fertility.

Keywords: Granulosa cells; Infertility; Irisin; Leptin; Ovary; PCR array.