The relation between NEAT1 expression level and survival rate in patients with oral squamous cell carcinoma

Nan-Chin Lin; Shih-Min Hsia; Tong-Hong Wang; Po-Jung Li; Yu-Hsin Tseng; Kuo-Chou Chiu; Hsi-Feng Tu; Yin-Hwa Shih; Tzong-Ming Shieh

doi:10.1016/j.jds.2021.09.018

The relation between NEAT1 expression level and survival rate in patients with oral squamous cell carcinoma

J Dent Sci. 2022 Jan;17(1):361-367. doi: 10.1016/j.jds.2021.09.018. Epub 2021 Sep 24.

Authors

Nan-Chin Lin^{1

2

3}, Shih-Min Hsia⁴, Tong-Hong Wang⁵, Po-Jung Li¹, Yu-Hsin Tseng⁶, Kuo-Chou Chiu⁷, Hsi-Feng Tu⁸, Yin-Hwa Shih⁹, Tzong-Ming Shieh¹

Affiliations

¹ School of Dentistry, College of Dentistry, China Medical University, Taichung, Taiwan.
² Department of Oral and Maxillofacial Surgery, Changhua Christian Hospital, Changhua, Taiwan.
³ Department of Oral and Maxillofacial Surgery, Show Chwan Memorial Hospital, Changhua, Taiwan.
⁴ School of Nutrition and Health Sciences, Taipei Medical University, Taipei, Taiwan.
⁵ Tissue Bank, Chang Gung University, Chang Gung Memorial Hospital, Taoyuan, Taiwan.
⁶ Department of Pediatrics, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan.
⁷ Division of Oral Diagnosis and Family Dentistry, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan.
⁸ Institute of Oral Biology, School of Dentistry, National Yang-Ming University, Taipei, Taiwan.
⁹ Department of Healthcare Administration, College of Medical and Health Science, Asia University, Taichung, Taiwan.

Abstract

Background/purpose: Numerous studies have shown that long noncoding RNAs (lncRNAs) are involved in cancer progression and chemotherapy resistance. Nuclear enriched abundant transcript 1 (NEAT1) is an lncRNA. It affects tumor cell progression and drug resistance in various tumors. However, the relation of NEAT1 and survival rate in oral squamous cell carcinoma (OSCC) requires further study.

Materials and methods: One normal gingival epithelium cell line, SG, three oral cancer cell lines (HSC3, OEC-M1, and SAS), 34 paired non-cancerous matched tissues (NCMT), and OSCC tissues were used in this study. Tri-reagent was used for total RNA extraction. NEAT1 expression was assessed by reverse transcription-quantitative PCR (RT-qPCR).

Results: NEAT1 expression in oral cancer cell lines was lower than that in normal cells and was significantly downregulated in OSCC. NEAT1 upregulation reduced the survival rate of patients with OSCC. NEAT1 upregulation also reduced the survival rate of OSCC patients treated with chemotherapy and radiotherapy.

Conclusion: These results indicate that NEAT1 expression is a valuable biomarker for the prediction and prognosis of oral cancer.

Keywords: Chemotherapy; Nuclear enriched abundant transcript 1 (NEAT1); Oral squamous cell carcinoma; Radiotherapy; Survival rate.