Description of Aureibaculum luteum sp. nov. and Aureibaculum flavum sp. nov. isolated from Antarctic intertidal sediments

Abstract

Two Gram-stain-negative, aerobic, non-motile, and rod-shaped bacterial strains, designated SM1352^T and A20^T, were isolated from intertidal sediments collected from King George Island, Antarctic. They shared 99.8% 16S rRNA gene sequence similarity with each other and had the highest sequence similarity of 98.1% to type strain of Aureibaculum marinum but < 93.4% sequence similarity to those of other known bacterial species. The genomes of strains SM1352^T and A20^T consisted of 5,108,092 bp and 4,772,071 bp, respectively, with the G + C contents both being 32.0%. They respectively encoded 4360 (including 37 tRNAs and 6 rRNAs) and 4032 (including 36 tRNAs and 5 rRNAs) genes. In the phylogenetic trees based on 16S rRNA gene and single-copy orthologous clusters (OCs), both strains clustered with Aureibaculum marinum and together formed a separate branch within the family Flavobacteriaceae. The ANI and DDH values between the two strains and Aureibaculum marinum BH-SD17^T were all below the thresholds for species delineation. The major cellular fatty acids (> 10%) of the two strains included iso-C_15:0, iso-C_15:1 G, iso-C_17:0 3-OH. Their polar lipids predominantly included phosphatidylethanolamine, one unidentified aminophospholipid, one unidentified aminolipid, and two unidentified lipids. Genomic comparison revealed that both strains possessed much more glycoside hydrolases and sulfatase-rich polysaccharide utilization loci (PULs) than Aureibaculum marinum BH-SD17^T. Based on the above polyphasic evidences, strains SM1352^T and A20^T represent two novel species within the genus Aureibaculum, for which the names Aureibaculum luteum sp. nov. and Aureibaculum flavum sp. nov. are proposed. The type strains are SM1352^T (= CCTCC AB 2014243 ^T = JCM 30335 ^T) and A20^T (= CCTCC AB 2020370 ^T = KCTC 82503 ^T), respectively.

Keywords: Antarctic intertidal sediments; Aureibaculum flavum sp. nov.; Aureibaculum luteum sp. nov.; Flavobacteriaceae; Polyphasic taxonomy; Polysaccharide utilization loci.