One-Dimensional Synergistic Core-Shell Nanozymes with Superior Peroxidase-like Activity for Ultrasensitive Colorimetric Detection of Blood Cholesterol

Hongzhen Peng; Jiaxing Zhang; Caixia Zeng; Chaoqun Zhou; Qingnuan Li; Na Lu; Lihua Wang

doi:10.1021/acsabm.0c00588

One-Dimensional Synergistic Core-Shell Nanozymes with Superior Peroxidase-like Activity for Ultrasensitive Colorimetric Detection of Blood Cholesterol

ACS Appl Bio Mater. 2020 Aug 17;3(8):5111-5119. doi: 10.1021/acsabm.0c00588. Epub 2020 Jul 20.

Authors

Hongzhen Peng^{1

2}, Jiaxing Zhang³, Caixia Zeng³, Chaoqun Zhou⁴, Qingnuan Li⁵, Na Lu³, Lihua Wang^{2

6}

Affiliations

¹ Division of Physical Biology and Bioimaging Center, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 201800, China.
² Shanghai Synchrotron Radiation Facility, Zhangjiang Laboratory, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China.
³ School of Materials Engineering, Shanghai University of Engineering Science, Shanghai 201620, China.
⁴ Pennsylvania State University, 128 Stamford Heights, State College, Pennsylvania 16803; United States.
⁵ Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
⁶ Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal University, 500 Dongchuan Road, Shanghai 200241, China.

PMID: 35021687
DOI: 10.1021/acsabm.0c00588

Abstract

In this paper, a simple and green strategy was proposed to fabricate one-dimensional core-shell Fe₃O₄@C/Ni nanocomposites. The rationally designed hybrid nanostructures notably exhibited an extremely excellent peroxidase-mimicking property, arising from the synergetic effects of Fe₃O₄, carbon, and Ni nanoparticles, along with the hollow and hierarchically porous nanostructures. Based upon the outstanding peroxidase-like activity and cholesterol oxidase cascade reaction, a label-free, ultrasensitive, and highly selective colorimetric assay for cholesterol determination has been developed. Under the optimized conditions, the colorimetric biosensor demonstrated a linear response to cholesterol ranging from 5 to 200 μM, with a relatively low detection limit of 0.17 μM. More importantly, cholesterol determination as low as 5 μM could be directly distinguished with the naked eye. In addition, we successfully determined the total cholesterol content in human serum samples with satisfactory accuracy and good precision. The Fe₃O₄@C/Ni nanocatalyst-based colorimetric biosensor provides great potential in point-of-care testing in disease diagnosis.

Keywords: cholesterol; colorimetric detection; nanozyme; one-dimensional; peroxidase-like; ultrasensitive.